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首页> 外文期刊>Sensors Journal, IEEE >A Nanobiosensor Based on 4-Hydroxyphenylpyruvate Dioxygenase Enzyme for Mesotrione Detection
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A Nanobiosensor Based on 4-Hydroxyphenylpyruvate Dioxygenase Enzyme for Mesotrione Detection

机译：基于4-羟基苯丙酮酸双加氧酶的纳米生物传感器用于甲基磺草酮的检测

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摘要

The herbicide residue from intensive agricultural activity provokes environmental disturbances and human health injuries. Among the enzymatic disruptor herbicides, mesotrione is able to inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD), which plays a key role in the carotenoid synthesis. Therefore, enzyme-based sensors are innovative options for monitoring herbicides used in agriculture. Compared to the standard sensors, biosensors have assorted advantages, such as practicality, quick response, low cost, and high sensitivity. A nanobiosensor was developed herein based on HPPD for mesotrione detection. Theoretically, the molecular docking and molecular dynamics simulation estimated the interacting regions of HPPD with mesotrione. Experimentally, the atomic force microscope tip functionalization with HPPD immobilized in self-assembled monolayers was confirmed by fluorescence microscopy and atomic force spectroscopy. The cross-linker N-(3-dimethylaminopropyl)--ethylcarbodiimide hydrochloride was responsible for properly preserving the enzyme on the tip. The nanobiosensor proposed here was successfully able to detect mesotrione molecules. Such effectiveness in the development of nanobiosensors promises reliable, precise, and low-cost techniques, which apply to a broad range of issues, from ecology to medicine.
机译：密集的农业活动产生的除草剂残留物会引起环境干扰和人类健康伤害。在酶促破坏性除草剂中，甲基磺草酮能够抑制4-羟苯基丙酮酸双加氧酶（HPPD），该作用在类胡萝卜素的合成中起关键作用。因此，基于酶的传感器是监测农业中使用的除草剂的创新选择。与标准传感器相比，生物传感器具有多种优点，例如实用性，快速响应，低成本和高灵敏度。本文基于HPPD开发了用于甲基磺草酮检测的纳米生物传感器。从理论上讲，分子对接和分子动力学模拟估计了HPPD与甲基磺草酮的相互作用区域。实验上，通过荧光显微镜和原子力光谱法证实了用固定在自组装单层中的HPPD进行的原子力显微镜尖端功能化。交联剂N-（3-二甲基氨基丙基）-乙基碳二亚胺盐酸盐负责正确保存尖端上的酶。本文提出的纳米生物传感器成功地能够检测甲基磺草酮分子。纳米生物传感器开发中的这种有效性保证了可靠，精确和低成本的技术，这些技术适用于从生态学到医学的广泛问题。

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