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首页> 外文期刊>Surface Engineering >Deposition of Yarrowia lipolytica on plasma prepared teflonlike thin films
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Deposition of Yarrowia lipolytica on plasma prepared teflonlike thin films

机译：血浆制备的聚四氟乙烯状薄膜上解脂耶氏酵母的沉积

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摘要

The adhesion of Yarrowia lipolytica to teflonlike thin films deposited by plasma on polycarbonate substrates was investigated through a series of tests in order to develop a substrate for strong and selective adhesion of Yarrowia lipolytica cells. Teflonlike thin films were prepared using atmospheric pressure surface barrier discharge with mixtures of octafluorocyclobutane (C4F8) and nitrogen as plasma gas. A variety of plasma gas feedrates and different deposition times were studied. The films were characterised by Fourier transform infrared and contact angle measurements using the sessile drop technique. Total surface energy and its components were calculated using the acid base theory. Attachment of the yeast cells was assessed by optical and scanning electron microscopy. The optimal deposition conditions for cell adhesion were determined using standard adhesion tests.
机译：通过一系列测试研究了解脂耶氏酵母对通过等离子体沉积在聚碳酸酯基底上的聚四氟乙烯薄膜的粘附力，从而开发了一种用于解脂耶氏酵母细胞的强而选择性粘附的基底。利用八氟环丁烷（C 4 F 8 ）和氮气的等离子混合物，通过大气压表面势垒放电制备特氟龙薄膜。研究了各种等离子气体进料速度和不同的沉积时间。使用固着滴技术通过傅里叶变换红外和接触角测量来表征薄膜。使用酸碱理论计算总表面能及其组分。通过光学和扫描电子显微镜评估酵母细胞的附着。使用标准粘附力测试确定细胞粘附的最佳沉积条件。

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