Penetration into Host Cells of Naked, Partially Injected (Post-FST) DNA of Bacteriophage T5

B. Labedan; J. Legault-Demare

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Penetration into Host Cells of Naked, Partially Injected (Post-FST) DNA of Bacteriophage T5

机译：渗透到裸，部分注射（后FST）DNA的宿主细胞T5

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The experiments presented here suggest that the naked post-FST DNA, attached to the bacteria after a centrifugation at 6,000 × g of Escherichia coli-T5 complexes arrested at the FST stage, can be injected into the host cell if protein synthesis is allowed. This results from the observation that the production of infectious centers by this naked DNA is sensitive to DNase or moderate shearing treatments. The process of injection seems to be the same as that for DNA normally rolled in an adsorbed capsid.

机译：此处提出的实验表明，在FST阶段被捕获的6,000× G -T5复合物以6,000× G / Em>在FST阶段被捕获的裸体后FST DNA。如果允许蛋白质合成，可以注射到宿主细胞中。这引出了这种观察结果，即这种裸体DNA的传染性中心的产生对DNase或中等剪切处理敏感。注射过程似乎与通常在吸附的衣壳中轧制的DNA的过程相同。 展开▼

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《Journal of Virology》 |1973年第2期|共4页

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B. Labedan; J. Legault-Demare;
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收录信息美国《科学引文索引》(SCI);

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1. Penetration into Host Cells of Naked, Partially Injected (Post-FST) DNA of Bacteriophage T5 [J] . B. Labedan, J. Legault-Demare Journal of Virology . 1973,第2期

机译：渗透到裸，部分注射（后FST）DNA的宿主细胞T5

2. Penetration into Host Cells of Naked, Partially Injected (Post-FST) DNA of Bacteriophage T5 [J] . B. Labedan, J. Legault-Demare Journal of Virology . 1973,第2期

机译：渗透到裸，部分注射（后FST）DNA的宿主细胞T5

3. Host cell metabolic energy is not required for injection of bacteriophage T5 DNA. [J] . A Filali Maltouf, B Labedan Journal of bacteriology . 1983,第1期

机译：注射噬菌体T5 DNA不需要宿主细胞代谢能。

4. Role of Nano-Chirality and Broken Symmetry in Hostile Organism Penetration of Host Cell DNA [C] . G.C. Vezzoli NSTI BioNano Conference and Trade Show . 2008

机译：纳米手性和破裂对称性在宿主细胞DNA渗透的作用

5. TWO BACTERIOPHAGE T4 GENE PRODUCTS WHICH REGULATE GENE EXPRESSION AFFECT DNA STRUCTURE: I. THE ALC GENE PRODUCT UNFOLDS THE HOST NUCLEOID. II. THE PSET GENE PRODUCT CAN "SHUTTLE" DNA PHOSPHATES FROM 3' TO 5' TERMINI. [D] . SIROTKIN, KARL M. 1978

机译：调节基因表达影响DNA结构的两种噬菌体T4基因产物：I. ALC基因产物使宿主核素折叠。二。 PSET基因产物可以“封闭” 3'至5'末端的DNA磷酸盐。

6. Penetration into Host Cells of Naked Partially Injected (Post-FST) DNA of Bacteriophage T5 [O] . B. Labedan, J. Legault-Demare 1973

机译：渗透到裸噬菌体T5的部分注射（FST后）DNA的宿主细胞中

7. THE ROLE OF CALCIUM IN THE PENETRATION OF BACTERIOPHAGE T5 INTO ITS HOST [O] . S. E. Luria, Doris L. Steiner 1954

机译：钙在噬菌体T5渗透到其宿主中的作用

8. Replication of Linear Duplex DNA in Vitro with Bacteriophage T5 DNA Polymerase [R] . Fujimura, R. K., Das, S. K., Allison, D. P., 1980

机译：用噬菌体T5 DNa聚合酶体外复制线性双链DNa

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1. 酯酶及其编码DNA和掺入此DNA的载体和宿主细胞 [P] . 中国专利： CN100467602C . 2009.03.11

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3. rna targeting dna; DNA polynucleotide; recombinant expression vector; genetically modified host cell in vitro; variant site-directed modification polypeptide; chimeric site-directed modification polypeptide; rna polynucleotide; genetically modified cell; transgenic non-human organism whose genome comprises a transgene; composition; site specific modification method of a target DNA; site specific transcriptional modulation method within a target DNA; site-specific modification method in target DNA; method of promoting site-specific cleavage and modification of a target DNA in a cell; method of producing a genetically modified cell in a subject; method of modifying target DNA in a genetically modified cell; kit; target dna target kit; method of selectively modulating transcription of a target DNA into a host cell; isolated nucleic acid; genetically modified host cell; and nucleic acid library [P] . 外国专利： BR112014029441A2 . 2017-06-27

机译：针对dna的rna; DNA多核苷酸;重组表达载体体外转基因宿主细胞;变体定点修饰多肽;嵌合定点修饰多肽; rna多核苷酸;转基因细胞基因组包含转基因的转基因非人类生物;组成;靶DNA的位点特异性修饰方法;靶DNA内的位点特异性转录调节方法;靶DNA的位点特异性修饰方法;促进细胞中靶DNA的位点特异性切割和修饰的方法;在受试者中产生基因修饰的细胞的方法;在转基因细胞中修饰靶DNA的方法;套件目标dna目标试剂盒;选择性调节靶DNA转录到宿主细胞中的方法;分离的核酸转基因宿主细胞;和核酸文库

4. Monoclonal or polyclonal antibody or equivalent ligand, use of the same, peptide, oligopeptide, polypeptide or protein, use of them, sequence of DNA, DNA or genomic DNA, bacteriophage clone, biologically functional plasmid or viral vector, host cell, processes for detection of a naturally occurring autoantibody, and of treatment [P] . 外国专利： BR9810118A . 2000-08-08

机译：单克隆或多克隆抗体或等效配体，相同的用途，肽，寡肽，多肽或蛋白质的使用，它们的使用，DNA，DNA或基因组DNA的序列，噬菌体克隆，生物学功能质粒或病毒载体，宿主细胞，检测方法天然自身抗体的制备及治疗

5. human cd37 binding antibody molecule; antibody; DNA molecule; expression vector; host cell; method for producing an antibody; pharmaceutical composition; cd37-expressing b-cell depletion method within a cell population; radioimmunoconjugate binding to human cd37; method of treating a b-cell malignancy; kit for the production of radioimmunoconjugate; and injectable preparation of chimeric or humanized hh1 antibody for use in pretreatment of a b-cell malignancy [P] . 外国专利： BR112014014258A2 . 2017-06-13

机译：人cd37结合抗体分子;抗体; DNA分子表达载体宿主细胞;产生抗体的方法;药物组合物;在细胞群中表达cd37的b细胞耗竭方法;放射免疫缀合物与人cd37的结合;治疗b细胞恶性肿瘤的方法;用于生产放射免疫缀合物的试剂盒;或人源化hh1抗体的可注射制剂，用于b细胞恶性肿瘤的预处理

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Penetration into Host Cells of Naked, Partially Injected (Post-FST) DNA of Bacteriophage T5

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