Isolation of membrane-bound renal enzymes that metabolize kinins and angiotensins

C D Gedney; E G Erd?s; P E Ward; R C Reynolds; R M Dowben

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Isolation of membrane-bound renal enzymes that metabolize kinins and angiotensins

机译：膜结合肾酶的分离，即代谢Kinins和血管紧张素

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pCortex of rat kidney was homogenized and fractions enriched in plasma membrane, endoplasmic reticulum or brush border were prepared by several techniques of differential centrifugation. The identity and homogeneity of the membrane fragments were investigated by assaying marker enzymes and by transmission and scanning electron microscopy. Kallikrein was present in both plasma-membrane- and endoplasmic-reticulum-enriched fractions isolated by two fractionation procedures. Kallikrein was highly concentrated in a plasma-membrane fraction but was absent from the brush-border membrane of proximal tubular cells. Cells of transplanted renal tumours of the rat, originating from the proximal tubule, had no kallikrein activity. Kininase activity, angiotensin I-converting enzyme (kininase II) and angiotensinase were found in a plasma-membrane-enriched fraction and especially in the fraction containing isolated brush border. It is suggested that after renal kallikrein is synthesized on endoplasmic reticulum, it is subsequently reoriented to a surface membrane for activation and release. Renal kallikrein may enter the tubular filtrate distal to the proximal tubules. The brush-border membrane of proximal tubule is the major site of inactivation of kinins and angiotensin II../p

机译：>大鼠肾的皮质是均质化的，并通过几种差分离心技术制备富含质膜，内质网或刷子边界的级分。通过测定标记酶和透射电子显微镜来研究膜片段的同一性和均匀性。 Kallikrein以两种分馏方法分离的血浆膜和内质 - 网状型富集的级分存在。 Kallikrein高度浓缩，膜膜馏分高，但不存在近端管状细胞的刷边膜。大鼠移植肾肿瘤的细胞，来自近端小管，没有Kallikrein活性。激素酶活性，血管紧张素I-转换酶（激酶II）和血管紧张素酶在富含血浆膜富集的级分中发现，特别是在含有隔离刷边界的级分中。建议在成内质网上合成肾kallikrein后，随后将其重新定向到用于活化和释放的表面膜。肾kallikrein可以进入远端小管的管状滤液。近端小管的刷界膜是Kinins和血管紧张素II灭活的主要部位..

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《The biochemical journal》 |1976年第3期|共页
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C D Gedney; E G Erd?s; P E Ward; R C Reynolds; R M Dowben;
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收录信息美国《科学引文索引》(SCI);
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1. Isolation of membrane-bound renal enzymes that metabolize kinins and angiotensins [J] . C D Gedney, E G Erd?s, P E Ward, The biochemical journal . 1976,第3期

机译：膜结合肾酶的分离，即代谢Kinins和血管紧张素
2. Isolation of membrane-bound renal enzymes that metabolize kinins and angiotensins [J] . C D Gedney, E G Erd?s, P E Ward, The biochemical journal . 1976,第3期

机译：膜结合肾酶的分离，即代谢Kinins和血管紧张素
3. Renal hemodynamics and renal kinins after angiotensin-converting enzyme inhibition [J] . Barbara H Clappison, Warwick P Anderson and Colin I Johnston Kidney international. . 1981,第5期

机译：血管紧张素转换酶抑制后的肾脏血流动力学和肾脏激肽
4. Impact of Angiotensin and Endothelin Converting Enzymes and Related Bradykinin on Renal Functions in L-NAME Hypertensive Rats [C] . Ali Zainal Omar, Ismail M. Maulood International Conference and Workshops on Basic and Applied Sciences . 2017

机译：血管紧张素和内皮素转化酶的影响及相关Bradykin对L型高血压大鼠肾功能的影响
5. ANGIOTENSIN CONVERTING ENZYME IN THE BRAIN, TESTIS, EPIDIDYMIS, PITUITARY GLAND AND ADRENAL GLAND (CAPTOPRIL, EUKEPHALIN CONVERTASE, PEPTIDASE, CORPUS STRIATUM, GUANIDINOETHYL MERCAPTOSUCCINIC ACID). [D] . STRITTMATTER, STEPHEN MARK. 1986

机译：大脑，睾丸，癫痫，垂体和肾上腺（CAPTOPRIL，EUKEPHALIN转化酶，PEPTIDASE，CORPUS STRIATUM，GUANIDINOETHYL MERCAPTOSUCCINICID酸）中的血管紧张素转化酶。
6. Isolation of membrane-bound renal enzymes that metabolize kinins and angiotensins. [O] . P E Ward, E G Erdös, C D Gedney, 1976

机译：分离代谢激肽和血管紧张素的膜结合肾酶。
7. Isolation of membrane-bound renal enzymes that metabolize kinins and angiotensins. [O] . Ward, P E, Erdös, E G, Gedney, C D, 1976

机译：分离代谢激肽和血管紧张素的膜结合肾酶。
8. Isolation of Membrane-Bound Renal Enzymes that Metabolize Kinins and Angtotensins. [R] . Ward, P. E., Erdos, E. G., Gedney, C. D., 1976

机译：分离代谢Kinins和angtotensins的膜结合肾酶。

Isolation of membrane-bound renal enzymes that metabolize kinins and angiotensins

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