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首页> 外文期刊>Journal of Virology >Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus-induced polypeptides.
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Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus-induced polypeptides.

机译：人巨细胞病毒感染细胞中蛋白质合成的序列：早期和晚期病毒诱导的多肽。

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摘要

At least 10 distinct early virus-induced polypeptides were synthesized within 0 to 6 h after infection of permissive cells with cytomegalovirus. These virus-induced polypeptides were synthesized before and independently of viral DNA replication. A majority of these early virus-induced polypeptides were also synthesized in nonpermissive cells, which do not permit viral DNA replication. The virus-induced polypeptides synthesized before viral DNA replication were hypothesized to be nonstructural proteins coded for by the cytomegalovirus genome. Their synthesis was found to be a sequential process, since three proteins preceded the synthesis of the others. Synthesis of all early cytomegalovirus-induced proteins was a transient process; the proteins reached their highest molar ratios before the onset of viral DNA replication. Late viral proteins were synthesized at the time of the onset of viral DNA replication, which was approximately 15 h after infection. Their synthesis was continuous and increased in molar ratios with the accumulation of newly synthesized viral DNA in the cells. The presence of the amino acid analog canavanine or azetadine during the early stage of infection suppressed viral DNA replication. The amount of viral DNA synthesis was directly correlated to the relative amount of late viral protein synthesis. Because synthesis of late viral proteins depended upon viral DNA replication, the proteins were not detected in permissive cells treated with an inhibitor of viral DNA synthesis or in nonpermissive cells that are restrictive for cytomegalovirus DNA replication.
机译：用细胞瘤病毒感染允许细胞后，在0至6小时内合成至少10个不同的早期病毒诱导的多肽。这些病毒诱导的多肽在病毒DNA复制之前和独立于病毒性DNA复制中合成。这些早期病毒诱导的多肽的大部分也在非疾病细胞中合成，不允许病毒DNA复制。在病毒DNA复制之前合成的病毒诱导的多肽被假设为由巨细胞病毒基因组编码的非结构蛋白。发现它们的合成是顺序过程，因为三种蛋白质在合成其他蛋白质之前。所有早期细胞病毒诱导的蛋白质的合成是瞬态过程;在病毒DNA复制开始前，蛋白质达到了最高的摩尔比。在病毒DNA复制的发生时合成晚期病毒蛋白，感染后约为15小时。它们的合成是连续的并且在摩尔比中增加了细胞中的新合成病毒DNA的摩尔比。在感染早期阶段的氨基酸类似物钙氰胺或偶氮内的存在抑制了病毒DNA复制。病毒DNA合成的量与晚期病毒蛋白合成的相对量直接相关。因为后期病毒蛋白的合成依赖于病毒DNA复制，所以在用病毒DNA合成抑制剂或在具有巨细胞病毒DNA复制的抑制性的非疾病细胞中未检测到蛋白质。

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    《Journal of Virology》 |1978年第3期|共16页
  • 作者

    M F Stinski;

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  • 收录信息 美国《科学引文索引》(SCI);
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