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首页> 外文期刊>Journal of Virology >Cell-free coupling of influenza virus RNA transcription and translation.
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Cell-free coupling of influenza virus RNA transcription and translation.

机译：流感病毒RNA转录和翻译的无细胞偶联。

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A cell-free coupled system for the transcription and translation of fowl plague virus RNA is described. The system utilizes a new nuclease-preincubated rabbit reticulocyte lysate that has a high sensitivity to exogenous mRNA and a very low level of nuclease activity. Translation of the viral proteins in the coupled system is strictly dependent upon the viral transcriptase activity. In the coupled system the optimal concentration of magnesium is intermediate between the optimum for transcription and that for translation. Translation of the viral proteins seems faithful. The products represent the major viral peptides M and NP and two peptides with the same electrophoretic mobility as HA and P2. Viron NA is not resolved in the kind of polyacrylamide gels described. Proteins M and NP were immunoprecipitable with monospecific antisera. It is concluded that the virion-associated RNA polymerase transcribes the negative-stranded segments of the viral genome coding for these major structural proteins into fully functional mRNA's.
机译：描述用于转录的无细胞耦合系统和鸟类悬浮病毒RNA的转录和翻译。该系统利用新的核酸酶 - 预孵育的兔网状细胞裂解物，其对外源mRNA具有高敏感性和非常低的核酸酶活性。耦合系统中的病毒蛋白的翻译严格依赖于病毒转录酶活性。在耦合系统中，镁的最佳浓度是转录最佳的中间体，并且用于翻译。病毒蛋白的翻译似乎忠诚。该产品代表主要病毒肽M和NP和具有与HA和P2相同电泳迁移率的两种肽。在所述聚丙烯酰胺凝胶中没有解决viron na。蛋白质M和NP与单特异性抗血清免疫沉淀。得出结论，病毒型相关的RNA聚合酶将对这些主要结构蛋白的病毒基因组的负链段转录为完全官能的mRNA。

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