Cell-free coupling of influenza virus RNA transcription and translation.

机译：流感病毒RNA转录和翻译的无细胞偶联。

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摘要

A cell-free coupled system for the transcription and translation of fowl plague virus RNA is described. The system utilizes a new nuclease-preincubated rabbit reticulocyte lysate that has a high sensitivity to exogenous mRNA and a very low level of nuclease activity. Translation of the viral proteins in the coupled system is strictly dependent upon the viral transcriptase activity. In the coupled system the optimal concentration of magnesium is intermediate between the optimum for transcription and that for translation. Translation of the viral proteins seems faithful. The products represent the major viral peptides M and NP and two peptides with the same electrophoretic mobility as HA and P2. Viron NA is not resolved in the kind of polyacrylamide gels described. Proteins M and NP were immunoprecipitable with monospecific antisera. It is concluded that the virion-associated RNA polymerase transcribes the negative-stranded segments of the viral genome coding for these major structural proteins into fully functional mRNA's.

机译：描述了一种用于禽瘟病毒RNA转录和翻译的无细胞偶联系统。该系统利用一种新的核酸酶预孵育兔网织红细胞裂解物，该裂解物对外源mRNA具有很高的敏感性，而核酸酶的活性却非常低。偶联系统中病毒蛋白的翻译严格取决于病毒转录酶的活性。在耦合系统中，镁的最佳浓度介于转录的最佳浓度和翻译的最佳浓度之间。病毒蛋白的翻译似乎是忠实的。产物代表主要的病毒肽M和NP，以及两种具有与HA和P2相同的电泳迁移率的肽。 Viron NA在所描述的聚丙烯酰胺凝胶类型中无法解决。蛋白M和NP可通过单特异性抗血清免疫沉淀。结论是，与病毒体相关的RNA聚合酶将编码这些主要结构蛋白的病毒基因组的负链片段转录为功能齐全的mRNA。

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期刊名称 Journal of Virology
作者
J Content; L D Wit; M Horisberger;
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年(卷),期 1977(22),2
年度 1977
页码 247–255
总页数 9
原文格式 PDF
正文语种
中图分类人体病毒学（致病病毒）;病毒（滤过性病毒）;
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专利

1. Cell-free coupling of influenza virus RNA transcription and translation. [J] . J Content, L D Wit, M Horisberger Journal of Virology . 1977,第2期

机译：流感病毒RNA转录和翻译的无细胞偶联。
2. Cell-free coupling of influenza virus RNA transcription and translation. [J] . J Content, L D Wit, M Horisberger Journal of Virology . 1977,第2期

机译：流感病毒RNA转录和翻译的无细胞偶联。
3. Cell-free coupling of influenza virus RNA transcription and translation. [J] . J Content, L D Wit, M Horisberger Journal of Virology . 1977,第2期

机译：流感病毒RNA转录和翻译的无细胞偶联。
4. Evaluation of antibody titer of the weaned pig following vaccination with a killed influenza A virus vaccine in the presence of maternal antibodies both pre and post heterologous influenza A virus cha [C] . Annual Meeting of the American Association of Swine Veterinarians . 2018

机译：在母体抗体存在下用杀死的流感疫苗疫苗接种后断奶猪的抗体滴度评价母体抗体的疫苗术前和后异源甲型血清血清血清
5. Replication of neurovirulent and non-neurovirulent human H1N1 influenza A viruses in mouse brain and nerve cell cultures: Virus strain-specific and host cell-dependent variations in progeny virus assembly,mRNA splicing efficiency, and expression of RNA gene segments 7 and 8 [D] . Husak, Paul James 1994

机译：在鼠脑和神经细胞培养物中复制神经毒力和非神经毒力的人类甲型H1N1流感病毒：子代病毒装配，mRNA剪接效率以及RNA基因片段7和8的表达中病毒株特异性和宿主细胞依赖性变异
6. Effect of UV irradiation on the expression of vaccinia virus gene products synthesized in a cell-free system coupling transcription and translation. [O] . W Bossart, D L Nuss, E Paoletti 1978

机译：紫外线辐射对痘苗病毒基因产物表达的影响该产物在无转录系统结合转录和翻译的过程中合成。
7. Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA [O] . J Content, L De Wit, M A Horisberger 1978

机译：在无细胞偶联系统中产生的病毒蛋白的肽测绘表征，用于流感病毒mRNA的转录和翻译

Cell-free coupling of influenza virus RNA transcription and translation.

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