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首页> 外文期刊>The biochemical journal >Bi- and tri-antennary human transferrin glycopeptides and their affinities for the hepatic lectin specific for asialo-glycoproteins
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Bi- and tri-antennary human transferrin glycopeptides and their affinities for the hepatic lectin specific for asialo-glycoproteins

机译：双抗长年人的人转移蛋白糖苷蛋白及其对Asialo-糖蛋白特异的肝凝集素的亲曲线

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pGlycopeptides were isolated from a proteolytic digest of human transferrin. After mild acid hydrolysis the desialylated glycopeptides were labelled by the galactose oxidase/NaBsup3/supHsub4/sub procedure and then fractionated by Sephadex-gel filtration or by anion-exchange chromatography. Either technique allowed separation of the two heterosaccharide chains (designated glycan I and glycan II) previously described for this protein by Spik, Vandersyppe, Fournet, Bayard, Charet, Bouquelet, Strecker & Montreuil (1974) (in iActes du Colloque Internationale No./i 221 vol. 1, pp. 483–499). Subsequent chromatography on Sepharose–concanavalin A separated fractions containing different quantities of carbohydrates for each glycan, as indicated by analyses. The isolated glycan fractions were then tested for their abilities to bind to the immobilized rabbit hepatic lectin. Our studies suggest that either glycan can have a bi- or tri-antennary structure. Desialylated biantennary glycans I and II did not bind to the hepatic lectin. Desialylated triantennary glycan I was slightly retarded by the hepatic lectin, whereas the triantennary glycan II consisted of equal quantities of a retarded and a bound type. Desialylated triantennary glycan II was totally displaced from the hepatic lectin by using a buffer containing 0.05m-EDTA. The results suggest that greater structural heterogeneity exists in the carbohydrate moiety of human transferrin than was previously envisaged. Such heterogeneity could be reflected in several molecular forms of human transferrin, which, after desialylation, differ significantly in their affinities for the hepatic lectin./p
机译：从人转移素的蛋白水解消化中分离出糖肽。在温和的酸性水解后，通过半乳糖氧化酶/ nab 3℃，然后通过Sephadex-凝胶过滤或通过阴离子交换色谱法分离或通过阴离子 - 交换色谱法将脱胶糖苷肽标记。通过Spik，Vandersyppe，Fournet，Bayard，Charet，Bouquelet，Strecker＆amp，允许使用前面描述的两种异辛糖链（指定的聚糖I和聚糖II）的两种异孢子链（指定的聚糖I和聚糖II）;蒙特鲁尔（1974年）（在追求Du Colloque Internationale No。 221卷。1，pp.483-499）。如分析所示，甲肾上腺素对琼脂糖 - 康丹林的分离级分含有不同量的碳水化合物。然后测试分离的聚糖级分，以使其能力与固定的兔肝凝集素结合。我们的研究表明，糖类可以具有双向或三长年结构。脱染的双炔糖甘蔗I和II没有与肝凝集素结合。肝凝集凝集素略微延迟了脱粘的三烯醛甘油，而三合一甘油II由相等的延迟和结合类型组成。使用含有0.05M-EDTA的缓冲液，从肝凝集素完全从肝凝集素中置换脱粘的三炔二。结果表明，在人转移素的碳水化合物部分中存在的结构异质性比以前设想的碳水化合物部分存在。这种异质性可以用几种分子形式的人转移素反射，在脱裂化之后，在它们的亲眼凝集素的亲眼症中显着不同。

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