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Relationship Among Tau Antigens Isolated from Various Lines of Simian Virus 40-Transformed Cells

机译：从辛安病毒40转化细胞中各种线分离的Tau抗原之间的关系

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In addition to the virus-specified tumor antigens, simian virus 40-transformed cells contain at least one other protein which can be immunoprecipitated with serum from animals bearing simian virus 40-induced tumors. This protein, which is designated Tau antigen, has an apparent molecular weight of 56,000 as determined by electrophoresis on acrylamide gels. The relationship among Tau antigens isolated from different lines of simian virus 40-transformed cells was examined by comparing the methionine-labeled tryptic peptides of these proteins by two-dimensional fingerprinting on thin-layer cellulose plates. In this fashion, we initially determined that the Tau antigens isolated from three different lines of transformed mouse cells were very similar. Second, we found that Tau antigen isolated from a line of rat transformants was closely related, but not identical, to the mouse cell Tau antigens. Approximately 70% of their methionine peptides comigrated in two dimensions. Finally, we showed that Tau antigen isolated from a line of transformed human cells was only partially related to the mouse and rat proteins. About 40% of the methionine peptides of the human protein were also contained in the Tau antigens from the other two species. These results strongly indicate that the Tau antigens isolated from these various simian virus 40-transformed cell lines contain common amino acid sequences.
机译：除了指定的病毒指定的肿瘤抗原之外，Simian病毒40变异的细胞含有至少一种其他蛋白质，其可以用含有Simian病毒40诱导的肿瘤的动物用血清免疫沉淀。该蛋白质指定Tau抗原，具有56,000的表观分子量，如丙烯酰胺凝胶上的电泳测定。通过在薄层纤维素板上比较了这些蛋白质的二维指纹蛋白标记的胰蛋白酶的甲硫氨酸标记的胰蛋白酶肽，通过二维指纹对薄层纤维素平板进行蛋白标记的胰蛋白酶肽来检查来自Simian病毒40转化细胞不同线的Tau抗原之间的关系。以这种方式，我们最初确定从三种不同的转化小鼠细胞中分离的Tau抗原非常相似。其次，我们发现从一系列大鼠转化体中分离的Tau抗原与小鼠细胞Tau抗原密切相关但不相同。大约70％的甲硫氨酸肽在两个维度中进行。最后，我们表明，从转化的人细胞中分离的Tau抗原仅部分与小鼠和大鼠蛋白质部分。来自其他两种物种的Tau抗原也包含约40％的人蛋白的甲硫氨酸肽。这些结果强烈表明，从这些各种猿猴病毒40转化的细胞系中分离的Tau抗原含有常见的氨基酸序列。

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