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首页> 外文期刊>Journal of Virology >Bacteriophage XP-12-induced exonuclease which preferentially hydrolyzes nicked DNA.
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Bacteriophage XP-12-induced exonuclease which preferentially hydrolyzes nicked DNA.

机译：噬菌体XP-12诱导的外切核酸酶，其优先水解切屑DNA。

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摘要

An exonuclease has been partially purified from XP-12-infected Xanthomonas oryzae which is not found in uninfected X. oryzae. Although both the phage-induced exonuclease and the major host exonucleolytic DNase released 5'-mononucleotides, these enzymes differed in their chromatographic behavior, pH optimum, salt inhibition, and heat sensitivity. These two exonucleases preferred different substrates. Nicked native DNA was the best substrate for the phage-induced enzyme, whereas denatured DNA was the best substrate for the host enzyme. Also, the host enzyme had a significant preference for denatured or nicked, normal cytosine-containing DNA (e.g., X. oryzae or T7 DNA) over similarly denatured or nicked 5-methylcytosine-rich DNA (namely, XP-12DNA), whereas the phage-induced enzyme hydrolyzed both types of DNA equally well.
机译：已经从XP-12感染的Xanthomonas oryzae部分纯化了外切核酸酶，该X氨基葡萄球菌未在未感染的X. Oryzae中发现。虽然噬菌体诱导的外切核酸酶和主要宿主外出核酸分解DNase释放了5'-单核苷酸，但这些酶在其色谱行为中不同，pH值最佳，盐抑制和热敏感性。这两个外切酶优选不同的基材。切口的天然DNA是噬菌体诱导的酶的最佳基材，而变性DNA是宿主酶的最佳底物。此外，宿主酶对变性或卷积的含量含量的含胞菌的DNA（例如，X. Oryzae或T7 DNA）具有显着的偏好，同样变性或富含5-甲基胞嘧啶的DNA（即XP-12DNA），而噬菌体诱导的酶均匀地水解两种类型的DNA。

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