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首页> 外文期刊>Journal of Virology >Genomic RNA of mengovirus. VI. Translation of its two cistrons in lysates of interferon-treated cells.
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Genomic RNA of mengovirus. VI. Translation of its two cistrons in lysates of interferon-treated cells.

机译：Mengovirus的基因组RNA。 VI。其两个传感在干扰素处理细胞的裂解物中的翻译。

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The addition of low levels (40 ng/ml) of the synthetic double-stranded polyribonucleotide poly I:C to lysates of interferon-treated L-cells resulted in a strong inhibition (70 to 75%) of the in vitro translation of mengovirus RNA. Under these conditions, the rates of incorporation of [35S]methionine or formyl-[35S]methionine were depressed to a comparable extent. The sequences of mengovirus RNA recognized by ribosomes of interferon-treated cells at initiation of translation were compared with those present in initiation complexes formed by ribosomes of untreated controls. Fingerprint analysis revealed that the same sequences of mengovirus RNA were protected against nuclease attack by the 80S and the 40S initiation complexes formed in vitro in lysates of control or interferon-treated L-cells. Mengovirus RNA-coded proteins were labeled at their N-terminal end with formyl-[35S]methionine and digested to completion with trypsin. The resulting fragments were separated by high-voltage paper electrophoresis. Two different formyl-[35S]methionine-labeled N termini were resolved. Further analyses supported the notion that the two radioactive peaks originated in the initiation of translation at two different sites. This pattern did not change when mengovirus RNA was translated in lysates of interferon-treated cells.
机译：将低水平（40ng / ml）的合成双链多核糖核苷酸多I：C加入干扰素处理的L细胞的裂解物，导致蒙皮病毒RNA的体外翻译的强抑制（70-75％） 。在这些条件下，将[35s]甲硫氨酸或甲酰胺掺入的速率抑制在可比程度上。将通过未处理对照的核糖体形成的引发络合物中存在的干扰素处理细胞的核糖病毒RNA序列进行了核糖体的序列。指纹分析显示，通过80s的80s和40S在对照或干扰素处理的L细胞中在体外形成的40S发起复合物的核病毒RNA的相同序列。用甲酰基 - [35s]甲硫氨酸在其N-末端标记Mengovirus RNA编码蛋白，并用胰蛋白酶消化完成。通过高压纸电泳分离所得片段。解析了两种不同的甲酰氨基 - [35s]标记的N末端。进一步分析支持这两个放射性峰起源于两个不同位点的翻译中的观念。当在干扰素处理细胞的裂解物中翻译Mengovirus RNA时，这种模式没有改变。

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