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首页> 外文期刊>Journal of Virology >Characterization of murine-specific leukemia virus receptor from L cells.
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Characterization of murine-specific leukemia virus receptor from L cells.

机译：L细胞鼠特异性白血病病毒受体的表征。

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The host cell receptor for Moloney murine leukemia virus was solubilized from murine L-cell membranes and characterized. In initial studies designed to identify a receptor-rich cell line, different mouse cells were screened for binding to Moloney gp70, the viral envelope glycoprotein which determines host cell-binding specificity. gp70 binding to murine L cells was specific and saturable, with an apparent affinity constant (Ka) of 4 X 10(8) M-1, and the number of receptors per cell (6 X 10(5)) was similar to that of other mouse fibroblast cell lines. Characterization of the gp70 receptor with regard to extraction by detergents, protease sensitivity, and heat denaturation suggests that the receptor is an intrinsic membrane protein. Upon extraction of L-cell membranes with 0.2% deoxycholic acid and precipitation with acetone, specific and saturable binding of gp70 could be detected. The solubilized gp70-binding component was eluted upon gel filtration on Sephacryl S-300 into a species with an approximate molecular weight of 110,000.
机译：莫尼鼠白血病病毒的宿主细胞受体从鼠L细胞膜中溶解并表征。在设计用于鉴定富含受体的细胞系的初始研究中，筛选不同的小鼠细胞以结合Moloney GP70，该母细胞糖蛋白确定宿主细胞结合特异性。 GP70与鼠L细胞的结合是特异性和可饱和的，表观亲和常数（Ka）为4×10（8）m-1，并且每个细胞的受体的数量相似与...类似的受体（6×10（5））其他小鼠成纤维细胞系。通过洗涤剂萃取的GP70受体的表征，蛋白酶敏感性和热变性表明受体是内在膜蛋白。用0.2％脱氧胆酸提取L细胞膜并用丙酮的沉淀，可以检测GP70的特异性和可饱和的结合。将溶解的GP70结合组分洗脱在凝胶过滤到梯度过滤到近似分子量为110,000的物质中。

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