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首页> 外文期刊>Weed technology >An in vivo Acetolactate Synthase Assay
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An in vivo Acetolactate Synthase Assay

机译：体内乙酰乳酸合酶测定

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A method was developed and tested for in vivo assay of acetolactate synthase (ALS). The method used foliar application of 1,1-cyclopropanedicarboxylic acid (CPCA) to inhibit ketol-acid reductoisomerase, the enzyme immediately following ALS in biosynthesis of branched-chain amino acids, thereby causing accumulation of acetolactate. Since the amount of acetolactate accumulation is a function of carbon flux through ALS, quantification of acetolactate accumulation determined ALS activity. Accumulation of acetolactate in soybean leaves resulted from CPCA rates as low as 15 g/ha and occurred within 1.5 h. Accumulation rates in soybean leaflets declined with leaf age from 84 μg/h/g tissue at 3 d to 17 μg/h/g tissue at 7 d. Foliar application of CPCA also caused acetolactate accumulation in corn, grain sorghum, velvetleaf, common cocklebur, and smooth pigweed. The ability of the in vivo assay to quantify the reduction in ALS activity following applications of ALS-inhibiting herbicides was validated by comparing ALS activity following thifensulfuron application to 'Williams 82' soybean, which has a sulfonylurea-sensitive ALS, and 'Asgrow 3200' soybean, which has a sulfonylurea-insen-sitive ALS. Thifensulfuron reduced ALS activity in Williams 82 soybean to 0, 0.8, 3.3, and 15.6% of the CPCA control at 6, 12, 24, and 48 HAT, but ALS activity in Asgrow 3200 soybean was reduced only to 34, 40, 57, and 88% of the CPCA control.
机译：开发了一种方法并测试了体内的乙酰乳酸合酶（ALS）。该方法通过叶面施用1,1-环丙烷二甲酸（CPCA）来抑制酮醇酸还原异构酶，酮醇酸还原异构酶是紧接在ALS之后的生物合成支链氨基酸的酶，从而引起乙酰乳酸的积累。由于乙酰乳酸积累的量是通过ALS的碳通量的函数，因此乙酰乳酸积累的定量决定了ALS的活性。 CPCA用量低至15 g / ha，并在1.5 h内发生，导致大豆叶片中乙酰乳酸的积累。随着叶龄的增长，大豆小叶中的积累速率从3 d时的84μg/ h / g组织下降到7 d时的17μg/ h / g组织。叶面喷施CPCA还会导致乙酰乳酸在玉米，高粱，天鹅绒，普通鸟蛤和光滑的杂草中积累。通过比较噻吩磺隆与具有磺酰脲敏感型ALS的“ Williams 82”大豆和“ Asgrow 3200”施用噻吩磺隆后的ALS活性，验证了体内测定法定量应用ALS抑制性除草剂后ALS活性降低的能力。大豆，具有对磺酰脲类药物敏感的ALS。噻吩磺隆在6、12、24和48 HAT时将Williams 82大豆中的ALS活性降低至CPCA对照的0、0.8、3.3和15.6％，但Asgrow 3200大豆中的ALS活性仅降低至34、40、57，和88％的CPCA控制。

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