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首页> 外文期刊>Pigment cell research >Studies of pigment transfer between Xenopus laevis melanophores and fibroblasts in vitro and in vivo
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Studies of pigment transfer between Xenopus laevis melanophores and fibroblasts in vitro and in vivo

机译：非洲爪蟾黑色素细胞与成纤维细胞之间的体内外色素转移研究

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Frog melanophores rapidly change colour by dispersion or aggregation of melanosomes. A long-term colour change exists where melanosomes are released from melanophores and transferred to surrounding skin cells. No in vitro model for pigment transfer exists for lower vertebrates. Frog melanophores of different morphology exist both in epidermis where keratinocytes are present and in dermis where fibroblasts dominate. We have examined whether release and transfer of melanosomes can be studied in a melanophore-fibroblast co-culture, as no frog keratinocyte cell line exists. Xenopus laevis melanophores are normally cultured in conditioned medium from fibroblasts and fibroblast-derived factors may be important for melanophore morphology. Melanin was exocytosed as membrane-enclosed melanosomes in a process that was upregulated by alpha-melanocyte-stimulating hormone (alpha-MSH), and melanosomes where taken up by fibroblasts. Melanosome membrane-proteins seemed to be of importance, as the cluster-like uptake pattern of pigment granules was distinct from that of latex beads. In vivo results confirmed the ability of dermal fibroblasts to engulf melanosomes. Our results show that cultured frog melanophores can not only be used for studies of rapid colour change, but also as a model system for long-term colour changes and for studies of factors that affect pigmentation.
机译：青蛙黑素细胞通过黑素体的分散或聚集迅速改变颜色。存在长期的颜色变化，其中黑素体从黑素体中释放出来并转移到周围的皮肤细胞中。对于低等脊椎动物，不存在用于色素转移的体外模型。在存在角质形成细胞的表皮和在成纤维细胞占主导的真皮中都存在不同形态的青蛙黑素细胞。我们已经检查了是否可以在黑素细胞-成纤维细胞共培养物中研究黑素体的释放和转移，因为不存在青蛙角质形成细胞系。非洲爪蟾黑色素细胞通常在成纤维细胞的条件培养基中培养，而成纤维细胞衍生的因子对于黑素细胞形态可能很重要。黑色素被膜封闭的黑色素体分泌，其过程被α-黑色素细胞刺激激素（α-MSH）上调，而黑色素体则被成纤维细胞吸收。黑素体膜蛋白似乎很重要，因为色素颗粒的团簇状摄取模式与乳胶珠不同。体内结果证实了真皮成纤维细胞吞噬黑素体的能力。我们的结果表明，培养的青蛙黑素细胞不仅可以用于快速颜色变化的研究，而且可以用作长期颜色变化的模型系统以及用于研究影响色素沉着的因素的模型系统。

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