...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Cell Reports >Characterization of a family of Arabidopsis receptor-like cytoplasmic kinases (RLCK class VI)
【24h】

Characterization of a family of Arabidopsis receptor-like cytoplasmic kinases (RLCK class VI)

机译：拟南芥受体样胞质激酶家族（RLCK VI类）的表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The receptor-like cytoplasmic protein kinases (RLCKs) are plant-specific proteins encoded by almost 200 genes in the Arabidopsis genome. Despite of their high number, the available information on the potential function of RLCKs is very limited. In this report, the sequence analysis and the gene expression pattern of 14 members of one of the Arabidopsis RLCK families (RLCK class VI) are described. Sequence comparison indicated that gene duplication played a significant role in the formation of the kinase family and that several members carry an N-terminal "universal stress protein" (UspA) domain. In order to gain insight into the potential function of the RLCK VI kinases, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to determine the relative transcript levels in the various organs of the Arabidopsis plant as well as under a series of abiotic stress/hormone treatments in seedlings. The obtained data revealed the differentially regulated expression of the genes in agreement with a high variability of sequence elements in their promoters. The divergent expression patterns indicate that the encoded kinase proteins may be involved in a wide variety of signal transduction pathways related to plant development and stress responses. The significance of gene duplication and expression divergence in the extension of the Arabidopsis RLCK VI family during evolution is discussed.
机译：受体样细胞质蛋白激酶（RLCKs）是拟南芥基因组中近200个基因编码的植物特异性蛋白。尽管数量众多，但有关RLCK潜在功能的可用信息非常有限。在此报告中，描述了拟南芥RLCK家族之一（RLCK VI类）的14个成员的序列分析和基因表达模式。序列比较表明基因重复在激酶家族的形成中起着重要作用，并且几个成员带有一个N末端的“普遍应激蛋白”（UspA）结构域。为了深入了解RLCK VI激酶的潜在功能，使用实时定量逆转录聚合酶链反应（qRT-PCR）来确定拟南芥植物各个器官以及在其下的相对转录水平。幼苗中的一系列非生物胁迫/激素处理。获得的数据揭示了基因的差异调节表达与启动子中序列元件的高度可变性一致。不同的表达模式表明，编码的激酶蛋白可能参与与植物发育和胁迫响应有关的多种信号转导途径。讨论了拟南芥RLCK VI家族进化过程中基因复制和表达差异的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号