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首页> 外文期刊>Plant Physiology and Biochemistry >Molecular cloning and expression of Chimonanthus praecox farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral volatile sesquiterpenoids
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Molecular cloning and expression of Chimonanthus praecox farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral volatile sesquiterpenoids

机译：蜡梅法尼基焦磷酸焦磷酸合酶基因的分子克隆和表达及其可能与花香挥发性倍半萜生物合成的关系

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摘要

Farnesyl pyrophosphate (FPP) synthase catalyzes the biosynthesis of FPP, which is the precursors of sesquiterpenoids such as floral scent volatiles, from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). cDNA encoding wintersweet (Chimonanthus praecox L.) FPP synthase was isolated by the RT-PCR and RACE methods. The deduced amino acid sequence showed a high identity to plant FPP synthases. Expression of the gene in Escherichia coli yielded FPPS activity that catalyzed the synthesis of FPP as a main product. Tissue-specific and developmental analyses of the mRNA levels of CpFPPS and volatile sesquiterpenoids levels in C. praecox flowers revealed that the FPPS may play a regulatory role in floral volatile sesquiterpenoids of wintersweet.
机译：法呢基焦磷酸酯（FPP）合酶催化FPP的生物合成，FPP是倍半萜类化合物的前体，例如花香挥发物，是由焦磷酸异戊烯基（IPP）和二甲基烯丙基焦磷酸酯（DMAPP）合成的。通过RT-PCR和RACE方法分离了编码冬甜（Chimonanthus praecox L.）FPP合酶的cDNA。推导的氨基酸序列显示出与植物FPP合酶的高度同一性。该基因在大肠杆菌中的表达产生了FPPS活性，该活性催化了FPP作为主要产物的合成。对C.praecox花中CpFPPS的mRNA水平和挥发性倍半萜含量进行组织特异性和发育分析表明，FPPS可能在冬甜的花朵挥发性倍半萜中起调节作用。

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