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首页> 外文期刊>Planta: An International Journal of Plant Biology >Preparative laser capture microdissection and single-pot cell wall material preparation: a novel method for tissue-specific analysis
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Preparative laser capture microdissection and single-pot cell wall material preparation: a novel method for tissue-specific analysis

机译：制备性激光捕获显微切割和单罐细胞壁材料制备：一种组织特异性分析的新方法

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摘要

In adaptation to their function the walls of plant cell display tissue-specific variations of composition according to their developmental stage, cell type and stress of various origin. It is therefore important to obtain a precise analytical data describing the cell wall composition with respect to these different factors. In the present work, laser capture microdissection (LCM) was used for isolating different tissues from the stem of Urtica dioica L. at a semi-preparative scale. The technique was associated for the first time to a one-pot sequential cell wall preparation and hydrolysis for the carbohydrate analysis of each cell type. The results demonstrate that the combination of LCM and micro-analytical methods can provide individual cell type composition and should improve our knowledge of the biochemical diversity of cell walls in plants. This approach will be of potential interest for the understanding of the effects of stress or genetic engineering on the composition of the cell walls.
机译：为了适应它们的功能，植物细胞壁根据它们的发育阶段，细胞类型和各种来源的压力表现出组织特定的组成变化。因此，重要的是要获得准确的分析数据，以描述有关这些不同因素的细胞壁组成。在目前的工作中，激光捕获显微切割术（LCM）被用于以半制备规模从荨麻叶的茎中分离出不同的组织。该技术首次与单罐顺序细胞壁制备和水解相关，用于每种细胞类型的碳水化合物分析。结果表明，LCM和显微分析方法的结合可以提供单个细胞类型的组成，并应提高我们对植物细胞壁生化多样性的认识。该方法对于了解应激或基因工程对细胞壁组成的影响可能具有潜在的意义。

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