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首页> 外文期刊>Biomaterials >Development of functionalized superparamagnetic iron oxide nanoparticles for interaction with human cancer cells.
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Development of functionalized superparamagnetic iron oxide nanoparticles for interaction with human cancer cells.

机译：用于与人类癌细胞相互作用的功能化超顺磁性氧化铁纳米粒子的开发。

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Our goal is to develop, characterize and optimize functionalized super paramagnetic iron oxide nanoparticles (SPION) demonstrating the capacity to be internalized by human cancer cells. SPION (mean diameter 9nm) were coated with various ratios to iron oxide of either polyvinyl alcohol (PVA), carboxylate-functionalized PVA, thiol-functionalized PVA and amino-functionalized PVA (amino-PVA). The interaction with cells and cytotoxicity of the SPION preparations were determined using human melanoma cells. From the four functionalized SPION preparations, only the amino-PVA SPION demonstrated the capacity to interact with, and were not cytotoxic to, human melanoma cells. This interaction with melanoma cells was dependent on the amino-PVA to iron oxide ratio, was an active and saturable mechanism displayed by all cells in a culture. These functionalized SPION were characterized by transmission electron microscopy and electrophoretic mobility. The physical comportment of SPION changed at specific PVAs to iron oxide ratios, and this ratio corresponded to the ratio of optimal interaction with cells. In conclusion, the successful development of functionalized SPION displaying potential cellular uptake by human cancer cells depends both on the presence of amino groups on the coating shell of the nanoparticles and of its ratio to the amount of iron oxide.
机译：我们的目标是开发，表征和优化功能化的超顺磁性氧化铁纳米粒子（SPION），以证明人类癌细胞内在化的能力。在聚乙烯醇（PVA），羧酸官能化的PVA，硫醇官能化的PVA和氨基官能化的PVA（氨基-PVA）的氧化铁上涂覆SPION（平均直径为9nm）。使用人黑素瘤细胞确定了SPION制剂与细胞的相互作用和细胞毒性。从四种功能化的SPION制剂中，只有氨基PVA SPION表现出与人黑素瘤细胞相互作用的能力，并且对人黑素瘤细胞没有细胞毒性。与黑色素瘤细胞的这种相互作用取决于氨基-PVA与氧化铁的比率，是培养物中所有细胞所表现出的活跃且可饱和的机制。这些功能化的SPION通过透射电子显微镜和电泳迁移率进行表征。 SPION的物理比例在特定的PVA与氧化铁比率下发生了变化，该比率对应于与细胞的最佳相互作用比率。总之，功能化SPION能否成功显示出人类癌细胞对细胞的摄取，取决于纳米颗粒涂层外壳上氨基的存在及其与氧化铁含量的比率。

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