...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Polimery >Application of derivatives of UV spectra and similarity indices to identification of milk proteins separated using reversed-phase high performance liquid chromatography
【24h】

Application of derivatives of UV spectra and similarity indices to identification of milk proteins separated using reversed-phase high performance liquid chromatography

机译：紫外光谱和相似指数的导数在反相高效液相色谱分离乳蛋白鉴定中的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The method which may be applied as a tool for identificatioin of proteins separated using reversed-phase high performance liquid chromatography, based on similarity indices of UV spectra derivatives, was proposed. Commercial milk protein preparations such as sodium and calcium caseinates, whey protein concentrate and milk protein coprecipitate as well as standards of milk proteins were used in the experiment. Statistical significance of difference between first and second similarity index, evaluated with Student's t test was used as a criterion to choose the mathematical treatment of spectra revealed the highest value of t parameter. The presence of statistically significant difference between first and second similarity indices always led to correct identification of proteins. The presented method enabled appropriate identification of caseins in commercial milk protein preparations, but failed in the case of whey proteins.
机译：提出了一种基于紫外光谱衍生物相似度指标的反相高效液相色谱分离蛋白鉴定方法。实验中使用了商业化的牛奶蛋白制品，例如酪蛋白酸钠和酪蛋白酸钠，乳清蛋白浓缩物和牛奶蛋白共沉淀物以及牛奶蛋白标准品。用学生t检验评估的第一和第二相似性指标之间差异的统计显着性用作选择光谱数学处理方法的标准，该方法揭示了t参数的最大值。第一和第二相似性指标之间存在统计学上的显着差异始终会导致正确鉴定蛋白质。所提出的方法能够对商业乳蛋白制品中的酪蛋白进行适当鉴定，但对于乳清蛋白则不能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号