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首页> 外文期刊>Polish Journal of Microbiology >Screening, characterization and biofilm formation of nickel resistant bacteria isolated from indigenous environment.
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Screening, characterization and biofilm formation of nickel resistant bacteria isolated from indigenous environment.

机译：从本地环境中分离出来的耐镍细菌的筛选，鉴定和生物膜形成。

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摘要

Nickel resistant bacteria (ZB, ZC, ZD, ZL, ZK and S1X) were isolated from industrial effluents and corroded iron pieces from indigenous environment of Punjab, Pakistan. These six strains could tolerate nickel at different levels with ZB, ZC, ZD, ZL, ZK, and S1X having 233, 225, 267, 233, 228 and 296 mM minimum inhibitory concentration (MIC) of nickel ions, respectively. These bacteria were sensitive to Cu(+2), Cr(+3), Co(+2), and Al(+3) as they did not grow even in the presence of 1 mM concentration of all these ions in minimal medium, whereas all of them were resistant to Fe3 upto 1.3 mM in minimal medium. The best appropriate temperature for nickel resistant bacteria was 37 degrees C and all of them showed maximum growth at pH 8. These bacteria were characterized morphologically and biochemically. Biofilm forming ability of the bacteria was checked with and without nickel stress and it was found that strains ZK and S1X were able to form a compact biofilm even under nickel stress. The sequencing of 16S rRNA-encoding genes from these nickel resistant bacteria showed that they belonged to four different genera namely, Klebsiella, Pseudomonas, Bacillus and Cronobacter.Registry Number/Name of Substance 0 (Industrial Waste). 0 (RNA, Ribosomal, 16S). 7OV03QG267 (Nickel).
机译：从工业废水和巴基斯坦旁遮普邦土著环境中腐蚀的铁片中分离出耐镍细菌（ZB，ZC，ZD，ZL，ZK和S1X）。这六个菌株分别具有分别具有233、225、267、233、233、228和296 mM镍离子最低抑制浓度（MIC）的ZB，ZC，ZD，ZL，ZK和S1X耐受不同水平的镍。这些细菌对Cu（+2），Cr（+3），Co（+2）和Al（+3）敏感，因为即使在最小培养基中所有这些离子的浓度为1 mM时它们也不会生长。而在基本培养基中，它们都对高达1.3 mM的Fe3具有抗性。最适合耐镍细菌的温度是37摄氏度，所有这些细菌在pH 8下均显示出最大的生长。这些细菌在形态和生化特征上都得到了表征。在有和没有镍胁迫下检查细菌的生物膜形成能力，并且发现菌株ZK和S1X即使在镍胁迫下也能够形成致密的生物膜。对这些抗镍细菌进行的16S rRNA编码基因测序表明，它们属于克雷伯菌，假单胞菌，芽孢杆菌和克罗诺杆菌四个不同属。注册号/物质0（工业废料）的名称。 0（RNA，核糖体，16S）。 7OV03QG267（镍）。

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