...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Phytochemical Analysis >Simultaneous quantification of 14 bioactive constituents in Forsythia Suspensa by liquid chromatography-electrospray ionisation-mass spectrometry
【24h】

Simultaneous quantification of 14 bioactive constituents in Forsythia Suspensa by liquid chromatography-electrospray ionisation-mass spectrometry

机译：液相色谱-电喷雾电离质谱法同时测定连翘中14种生物活性成分的含量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction - Forsythia suspensa is a commonly used traditional Chinese medicine including phenylethanoid glycosides, lignans, flavonoids, terpenes and volatile oils. Quantification of multi-components is important for the quality control of Forsythia suspensa.Objective - To establish a liquid chromatography-electrospray ionisation-mass spectrometry method for simultaneous quantification of 14 bioactive constituents of Forsythia suspensa in different places of China and different parts of this herb.Methodology - The optimal chromatographic conditions were achieved on a Kromasil C column (150 [yen sign] 4.6 mm, 5 mm) with gradient elution of methanol, acetonitrile and 0.1% formic acid in 27 min. Detection was performed in negative ionisation mode by monitoring the precursor-product combination in multiple reaction monitoring (MRM) mode. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability and accuracy.Results - All calibration curves showed good linear regression (r > 0.9990) within test ranges. The established method showed good precision and accuracy with overall intra-day and inter-day variations of 0.7-4.3 and 1.1-3.9% respectively, and overall recoveries of 96.65-101.2% for the compounds analysed.Conclusion - The proposed method was successfully applied for the quantitative analysis of 14 constituents in 12 Forsythia suspensa samples
机译：简介-连翘是一种常用的中药，包括苯乙醇类苷，木脂素，类黄酮，萜烯和挥发油。目的-建立液相色谱-电喷雾电离质谱联用技术同时测定中国不同地方和该药材不同部位连翘中14种生物活性成分的方法。方法学-最佳色谱条件是在Kromasil C色谱柱（150 [yen sign] 4.6 mm，5 mm）上用甲醇，乙腈和0.1％甲酸在27分钟内进行梯度洗脱。通过在多反应监测（MRM）模式下监测前体-产物组合，以负电离模式进行检测。方法的验证包括线性，灵敏度，精密度，可重复性，稳定性和准确性的测试。结果-所有校准曲线在测试范围内均表现出良好的线性回归（r> 0.9990）。建立的方法具有良好的精密度和准确度，日间和日间总变化分别为0.7-4.3和1.1-3.9％，所分析化合物的总回收率为96.65-101.2％。结论-该方法已成功应用用于12个连翘样品中14种成分的定量分析

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号