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首页> 外文期刊>Phytochemical Analysis >Analysis of ellagic acid in pomegranate rinds by capillary electrophoresis and high-performance liquid chromatography
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Analysis of ellagic acid in pomegranate rinds by capillary electrophoresis and high-performance liquid chromatography

机译：毛细管电泳-高效液相色谱法分析石榴皮中鞣花酸的含量

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摘要

Two novel methods for the analysis of ellagic acid in pomegranate (Punica granatum) rinds are proposed. Capillary electrophoresis (CE) was performed in a bare fused-silica capillary using a buffer solution of tri(hydroxymethyl)aminomethane:potassium dihydrogen phosphate (pH 8.4) with an applied voltage of 20 kV and UV detection at 254 nm. HPLC analysis was performed with a Zobax SB C(18) column and a mobile phase consisting of methanol:ethyl acetate:potassium dihydrogen phosphate: phosphoric acid at a flow rate of 1.0 mL/min. Under optimised conditions, the HPLC retention and the CE migration times for ellagic acid were 10.32 and 12.23 min, respectively. Calibration curves of peak area vs. concentration gave correlation coefficients of 0.9999 for HPLC and 0.9990 for CE. The detection limits for HPLC and CE were 2.8 and 2.2 microg/mL, respectively. Average recoveries were 98.32 +/- 1.2% for HPLC and 96.52 +/- 2.8% for CE. Both methods were shown to be suitable for the determination of ellagic acid in pomegranate rinds extraction; however, the CE method required less solvent and gave better column efficiency, whilst the HPLC provided superior precision. Copyright (c) 2008 John Wiley & Sons, Ltd.
机译：提出了两种新颖的方法来分析石榴皮中鞣花酸的含量。毛细管电泳（CE）在裸露的熔融石英毛细管中，使用三（羟甲基）氨基甲烷：磷酸二氢钾（pH 8.4）的缓冲溶液，施加电压20 kV，并在254 nm处进行UV检测。用Zobax SB C（18）色谱柱和流动相（以甲醇：乙酸乙酯：磷酸二氢钾：磷酸）为流动相，以1.0 mL / min的流速进行HPLC分析。在最佳条件下，鞣花酸的HPLC保留时间和CE迁移时间分别为10.32和12.23分钟。峰面积与浓度的校正曲线给出的相关系数对于HPLC为0.9999，对于CE为0.9990。 HPLC和CE的检出限分别为2.8和2.2 microg / mL。 HPLC的平均回收率为98.32 +/- 1.2％，CE的平均回收率为96.52 +/- 2.8％。两种方法均适用于石榴皮提取物中鞣花酸的测定。但是，CE方法所需的溶剂更少，色谱柱效率更高，而HPLC提供了更高的精度。版权所有（c）2008 John Wiley＆Sons，Ltd.

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