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首页> 外文期刊>Phytochemical Analysis >Determination of macrocyclic ellagitannin oenothein B in plant materials by HPLC-DAD-MS: method development and validation.
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Determination of macrocyclic ellagitannin oenothein B in plant materials by HPLC-DAD-MS: method development and validation.

机译：HPLC-DAD-MS测定植物材料中的大环鞣花鞣质蛇皮苷B：方法开发与验证。

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摘要

Introduction - Oenothein B (OeB) is a dimeric macrocyclic ellagitannin occurring mainly in the genus Oenothera. In the literature no validated HPLC method for quantification of this compound has been reported. Objective - To develop and validate an efficient HPLC method for quantification of OeB in plant materials. Methodology - Extraction conditions of OeB from plant material were optimised. Then the first validated HPLC method for quantification was developed. Results - We have shown that ultrasonic-assisted extraction at 40 degrees C in 15 min using water are the most optimal conditions for OeB extraction. We have quantified OeB in several plant materials belonging to Oenotheraceae family. The lowest amount of OeB was present in Circaea lutetiana herb (27.64+or-0.26 mg/g) and the highest was quantified in Epilobium hirsutum aerial parts (72.91+or-1.38 mg/g). Conclusion - The first HPLC method for quantification of OeB in plant materials was developed and validated. We also for the first time optimised the extraction conditions for OeB.
机译：简介-Oenothein B（OeB）是主要存在于Oenothera属的二聚体大环鞣花单宁。在文献中，没有报道用于该化合物定量的经验证的HPLC方法。目的-开发和验证一种有效的HPLC方法，用于定量植物材料中的OeB。方法-优化了从植物材料中提取OeB的条件。然后开发了第一种经过验证的高效液相色谱法进行定量。结果-我们已经表明，用水在40摄氏度下15分钟内超声辅助提取是OeB提取的最佳条件。我们已经对属于月见草科的几种植物中的OeB进行了定量。最低限度的OeB含量存在于小节瓜中（27.64+或-0.26 mg / g），而最高的定量存在于陆地上叶（Epilobium hirsutum）地上部分（72.91+或-1.38 mg / g）。结论-开发并验证了第一种用于定量植物材料中OeB的HPLC方法。我们还首次优化了OeB的提取条件。

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