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首页> 外文期刊>Poultry Science >Molecular identification of avian leukosis virus subgroup E loci and tumor virus B locus in Chinese indigenous chickens
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Molecular identification of avian leukosis virus subgroup E loci and tumor virus B locus in Chinese indigenous chickens

机译：中国本土鸡禽白血病病毒亚基因E基因座和B病毒基因座的分子鉴定

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摘要

Avian leukosis virus (ALV) subgroup E (ALVE) is an endogenous retrovirus in the chicken genome. The chickens carrying ALVE locus 3 (ALVE3), 6 (ALVE6), 9 (ALVE9), and 21 (ALVE21) have been proved to be susceptible to ALV. Tumor virus locus B (TVB) encodes the cellular receptor for ALV subgroups B, D, and E. The insertions of the 4 ALVE loci and the genotypes of TVB have not been demonstrated in Chinese indigenous chicken breeds. In the present study, the existence of ALVE3, ALVE6, ALVE9, and ALVE21 were detected in 10 native breeds of Chinese chickens and an introduced breed, the White Leghorn (2 populations in this study, WL1 and WL2), by locus-specific PCR. The PCR products of ALVE were further confirmed by sequencing assay. We also surveyed the status of genotypes of TVB in Si Me, Beijing You, and White Leghorn (WL1 and WL2) chickens with pyrosequencing assays. The results showed that the carrier frequency of ALVE3 was 1.3% in the Chinese chicken population, and was 10.3 in WL1 and 49.2% in WL2. The carrier frequency of ALVE6 was 5.4% in native breeds of Chinese birds, in contrast with 0% in WL1 and 6.8% in WL2. The carrier frequency of ALVE.9 was 0.1% in the Chinese indigenous population, and was 16.0% in WL1 and 11.9% in WL2. The carrier frequency of ALVE21 was 10.4% in Chinese chickens, whereas ALVE21 was detected with a frequency of 0% in WL1 and 50% in WL2. The frequency of the TVB resistance allele ( TVB*R and TVB*R') was 0.4% in Beijing You chickens, whereas it was 70.5% in WL1 and 54.5% in WL2. No carriers of ALVE3, ALVE9, and ALVE21 were detected in Silkie fowl, a famous Chinese native breed that has been used as a source for alternative medicine. These results present molecular evidence of ALVE3, ALVE6, ALVE9, and ALVE21 insertions and TVB genotypes in Chinese indigenous chickens and could provide potential molecular insights into anti-ALV breeding in chickens.
机译：禽白血病病毒（ALV）E亚组（ALVE）是鸡基因组中的内源性逆转录病毒。已证明携带ALVE基因座3（ALVE3），6（ALVE6），9（ALVE9）和21（ALVE21）的鸡对ALV敏感。肿瘤病毒基因座B（TVB）编码ALV亚组B，D和E的细胞受体。在中国本土鸡种中尚未证明4个ALVE基因座的插入和TVB的基因型。在本研究中，通过基因座特异性PCR在10个中国本土鸡品种和一个引入品种White Leghorn（本研究中有2个种群，WL1和WL2）中检测到了ALVE3，ALVE6，ALVE9和ALVE21的存在。 。通过测序测定进一步证实了ALVE的PCR产物。我们还通过焦磷酸测序法调查了Si Me，Beijing You和White Leghorn（WL1和WL2）鸡中TVB基因型的状况。结果表明，ALVE3的载波频率在中国鸡群中为1.3％，在WL1中为10.3，在WL2中为49.2％。在中国本土鸟类中，ALVE6的载波频率为5.4％，而WL1为0％，WL2为6.8％。 ALVE.9的载波频率在中国土著人口中为0.1％，在WL1中为16.0％，在WL2中为11.9％。在中国鸡中，ALVE21的载波频率为10.4％，而在WL1中检测到的ALVE21频率为0％，在WL2中检测到的频率为50％。 TVB耐药等位基因（TVB * R和TVB * R'）的频率在北京有鸡中为0.4％，而在WL1中为70.5％，在WL2中为54.5％。在中国著名的本地品种“乌贼”中未检测到ALVE3，ALVE9和ALVE21的携带者。这些结果提供了中国本土鸡中ALVE3，ALVE6，ALVE9和ALVE21插入以及TVB基因型的分子证据，并且可以为鸡抗ALV育种提供潜在的分子见解。

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