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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Sequence analysis of the plasmid genome of the probiotic strain Lactobacillus paracasei NFBC338 which includes the plasmids pCD01 and pCD02
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Sequence analysis of the plasmid genome of the probiotic strain Lactobacillus paracasei NFBC338 which includes the plasmids pCD01 and pCD02

机译：益生菌副干酪乳杆菌NFBC338的质粒基因组的序列分析，其包括质粒pCD01和pCD02

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摘要

Lactobacillus paracasei NFBC338 is a probiotic strain that was isolated from the human gastrointestinal tract (GIT) and contains a plasmid genome of >= 80 kb. Using a shotgun sequencing approach, two of the plasmids, pCD01 (19,882 bp) and pCD02 (8554bp) have been completely sequenced, and four contiguous sequences (Contigs) have been assembled. Bioinformatic analysis. of pCD01 revealed that it contains 23 putative open reading frames (ORFs) and that it contains regions characterised by potential replication functions and multidrug resistance (MDR). In contrast, the content of pCD02 is mainly cryptic, although, it does contain two insertion sequence (IS) elements. Indeed, up to 17% of the entire plasmid genome encodes putative transposable elements. In addition, there are a number of interesting ORFs distributed over the four Contigs that show significant homology to genes such as those involved in adherence and biotin metabolism, which may prove beneficial to Lb. paracasei NFBC338 under certain environmental conditions. This study provides a novel insight into the rich plasmid complement of this probiotic Lactobacillus strain, which may potentially be exploited as the basis for development of improved genetic tools for probiotic lactobacilli. (c) 2005 Published by Elsevier Inc.
机译：副干酪乳杆菌NFBC338是从人胃肠道（GIT）分离的益生菌菌株，并含有≥80kb的质粒基因组。使用a弹枪测序方法，已对两个质粒pCD01（19,882 bp）和pCD02（8554bp）进行了完整测序，并已装配了四个连续序列（Contigs）。生物信息学分析。 pCD01的图谱显示其包含23个推定的开放阅读框（ORF），并且包含以潜在的复制功能和多药耐药性（MDR）为特征的区域。相反，pCD02的内容主要是隐秘的，尽管它确实包含两个插入序列（IS）元素。实际上，整个质粒基因组中多达17％的基因编码推定的转座因子。此外，在四个重叠群上分布着许多有趣的ORF，这些ORF与与粘附和生物素代谢相关的基因显示出显着的同源性，这可能对Lb有益。副干酪NFBC338在某些环境条件下。这项研究为这种益生菌乳杆菌菌株的丰富质粒补体提供了新的见解，它可能被潜在地用作开发改进的益生菌乳杆菌遗传工具的基础。 （c）2005年由Elsevier Inc.发布。

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