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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >CHARACTERIZATION OF PBP614, A PUTATIVE ROLLING-CIRCLE PLASMID FROM BACILLUS POPILLIAE
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CHARACTERIZATION OF PBP614, A PUTATIVE ROLLING-CIRCLE PLASMID FROM BACILLUS POPILLIAE

机译：PBP614的鉴定，是一种来自灰质芽孢杆菌的滚动性圆形疟原虫

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摘要

A plasmid, pBP614 (5.647 kb), has been isolated from the New Zealand Bacillus popilliae strain 17 and characterized by physical mapping, cloning, and sequencing. An open reading frame was found which could encode a protein with homology to the replication (Rep) proteins of rolling-circle plasmids. The predicted B. popilliae Rep protein shows closest homology to Rep proteins from Bacillus plasmids of the pC194 family. Sequences homologous to plus- and minus-strand replication origins were found. The minus-strand origin shows similarities to the pal-T type family. Taken together, these observations suggest that pBP614 is a rolling-circle plasmid, the first such plasmid characterized from B. popilliae. (C) 1997 Academic Press. [References: 32]
机译：从新西兰芽孢杆菌17株中分离出质粒pBP614（5.647kb），并通过物理作图，克隆和测序来表征。发现一个开放阅读框，其可以编码与滚环质粒的复制（Rep）蛋白具有同源性的蛋白质。预测的流行芽孢杆菌Rep蛋白显示出与来自pC194家族的芽孢杆菌质粒的Rep蛋白最接近的同源性。发现与正链和负链复制起点同源的序列。负链起源与pal-T型家族相似。综上所述，这些观察结果提示pBP614是滚环质粒，这是首个以粟酒芽孢杆菌为特征的质粒。 （C）1997学术出版社。 [参考：32]

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