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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Kluyveromyces lactis killer plasmid pGKL2: evidence for a viral-like capping enzyme encoded by ORF3.
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Kluyveromyces lactis killer plasmid pGKL2: evidence for a viral-like capping enzyme encoded by ORF3.

机译：乳酸克鲁维酵母杀手质粒pGKL2：由ORF3编码的病毒样加帽酶的证据。

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摘要

ORF3 of the cytoplasmic linear plasmid pGKL2 was disrupted in vivo by integration of a selectable marker. Long-term cultivation of transformants carrying hybrid plasmids with a disrupted ORF3 under selective pressure did not deprive strains of the native counterpart, thereby proving its essentiality for pGKL2 replication and maintenance. The predicted ORF3 polypeptide was found to contain conserved motifs acquainted with mRNA-capping enzymes in the required order, just as in cytoplasmic viruses; new conserved motifs were also identified. Copyright 1998 Academic Press.
机译：细胞质线性质粒pGKL2的ORF3在体内通过整合选择标记而被破坏。在选择性压力下长期培养携带带有破坏的ORF3的杂种质粒的转化体，并没有使菌株失去天然的对应物，从而证明了其对于pGKL2复制和维持的必要性。发现预测的ORF3多肽含有所需顺序的保守的基序，这些基序与mRNA封端酶相似，就像在细胞质病毒中一样。还确定了新的保守基序。版权所有1998学术出版社。

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