Improved plasmid-based system for fully regulated off-to-on gene expression in Escherichia coli: Application to production of toxic proteins

CronanJ.E.

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Improved plasmid-based system for fully regulated off-to-on gene expression in Escherichia coli: Application to production of toxic proteins

机译：改进的基于质粒的系统，用于大肠杆菌中完全调控的即兴基因表达：在生产有毒蛋白质中的应用

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In previous work transduction of Escherichia coli with phage λ particles carrying packaged plasmids was shown to provide complete off-to-on expression of plasmid-borne genes (Cronan, J.E., 2003. J. Bacteriol. 185, 6522-6529). The plasmids used contained the phage λ. cos site (and hence are cosmids) and were very efficiently packaged into λ phage particles in vivo upon induction of λ lysogens having an inactivated cos site. However, a shortcoming was that the stocks contained a variable fraction of infectious λ phage which arose by recombinational repair of the inactive cos site. I report that the construction of E. coli strains that eliminate the background of infectious phage and show that the system can be utilized to express proteins by the phage T7 RNA polymerase/pET vector system.

机译：在以前的工作中，显示带有携带包装的质粒的噬菌体λ颗粒的大肠杆菌的转导提供了质粒携带的基因的完整表达（Cronan，J.E.，2003. J. Bacteriol。185，6522-6529）。所使用的质粒包含噬菌体λ。 cos位点（因此是粘粒），在诱导具有灭活的cos位点的λ溶原菌后，在体内非常有效地包装到λ噬菌体颗粒中。但是，缺点是这些种群包含可变比例的传染性λ噬菌体，这些噬菌体是通过对无活性的cos位点进行重组修复而产生的。我报告说，大肠杆菌菌株的构建消除了感染性噬菌体的背景，并表明该系统可用于噬菌体T7 RNA聚合酶/ pET载体系统表达蛋白质。

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《Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems》 |2013年第1期|共9页
作者
CronanJ.E.;
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正文语种 eng
中图分类医学遗传学;
关键词
Cosmid; DNA packaging; Phage lambda; Protein expression;

机译：粘粒;DNA包装;噬菌体λ;蛋白质表达;

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1. Improved plasmid-based system for fully regulated off-to-on gene expression in Escherichia coli: Application to production of toxic proteins [J] . CronanJ.E. Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 2013,第1期

机译：改进的基于质粒的系统，用于大肠杆菌中完全调控的即兴基因表达：在生产有毒蛋白质中的应用
2. Comparative Transcription Profiling and In-Depth Characterization of Plasmid-Based and Plasmid-Free Escherichia coli Expression Systems under Production Conditions [J] . Juergen Mairhofer, Theresa Scharl, Karoline Marisch, Applied and Environmental Microbiology . 2013,第12期

机译：在生产条件下基于质粒和无质粒的大肠杆菌表达系统的比较转录分析和深度表征
3. Comparative Transcription Profiling and In-Depth Characterization of Plasmid-Based and Plasmid-Free Escherichia coli Expression Systems under Production Conditions [J] . Juergen Mairhofer, Theresa Scharl, Karoline Marisch, Applied Microbiology . 2013,第12期

机译：在生产条件下基于质粒和无质粒的大肠杆菌表达系统的比较转录分析和深度表征
4. AN EXTRACELLULAR PROTEIN EXPRESSION SYSTEM IN ESCHERICHIA COLI IMPLIES POTENTIAL APPLICATION [C] . Qingsheng Qi, Junshu Wang, Dongfang Gao, Enzyme Engineering Conference . 2018

机译：大肠杆菌中的细胞外蛋白表达系统意味着潜在的应用
5. Characterization of the ARC1 gene of Saccharomyces cerevisiae and development of a high-yield expression system for the production of the cyanobacterial phytochrome Cph1 in Escherichia coli. [D] . Santos, Jennifer Lynn. 2002

机译：酿酒酵母的ARC1基因的表征和高产表达系统的发展，用于在大肠杆菌中生产蓝细菌植物色素Cph1。
6. Improved Plasmid-Based System for Fully Regulated Off-To-On Gene Expression in Escherichia coli: Application to Production of Toxic Proteins [O] . John E. Cronan -1

机译：改善基于质粒的系统用于大肠杆菌的完全核对基因表达：毒性蛋白的生产应用
7. Improved plasmid-based system for fully regulated off-to-on gene expression in Escherichia coli: Application to production of toxic proteins [O] . John E. Cronan 2013

机译：改善基于质粒的系统，用于大肠杆菌的完全核对基因表达：毒性蛋白的生产应用
8. Expression in Escherichia coli K-12 of the 76, 000-Dalton Iron-Regulated Outer Membrane Protein of Shigella flexneri Confers Sensitivity to Cloacin DF13 in the Absence of Shigella O Antigen. (Reannouncement with New Availability Information). [R] . Derbyshire, U., Baldwin, T., Stevenson, P., 1989

机译：在福氏志贺菌的76,000道尔顿铁调节的外膜蛋白的大肠杆菌K-12中的表达赋予在没有志贺氏菌抗原的情况下对Cloacin DF13的敏感性。（重新公布新的可用性信息）。

Improved plasmid-based system for fully regulated off-to-on gene expression in Escherichia coli: Application to production of toxic proteins

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