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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Sequence analysis of the plasmid pRR12 from the rumen bacterium Prevotellaruminicola 223/M2/7 and the use of pRR12 in Prevotella/Bacteroides shuttlevectors
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Sequence analysis of the plasmid pRR12 from the rumen bacterium Prevotellaruminicola 223/M2/7 and the use of pRR12 in Prevotella/Bacteroides shuttlevectors

机译：瘤胃细菌Prevotellaruminicola 223 / M2 / 7的质粒pRR12的序列分析以及pRR12在Prevotella / Bacteroides穿梭载体中的应用

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摘要

pRRI2 is a small cryptic plasmid from the rumen bacterium Prevotella ruminicola 223/M2/7 which has been used for the construction of shuttle vectors (pRH3 and pRRI207) that replicate in many Bacteroides/Prevotella strains as well as in Escherichia coli. Sequence analysis of pRRI2 reveals that it is a 3240-bp plasmid carrying two clear open reading frames. Rep. encoded by ORF1. shows 48 and 47% amino acid sequence identity with RepA proteins from Bacteroides vulgatus and Bacteroides fragilis. respectively. ORF2, named Pre, shares 34% amino acid sequence identity with a putative plasmid recombination protein from the Flavobacterium spp. plasmid pFL1 and 30% amino acid sequence identity with BmpH from B. fragilis Tn5520. Disruption of ORF1 with HindIII prevents repli cation and maintenance in Bacteroides spp. hosts, but shuttle vectors carrying pRRI2 interrupted within ORP2. by EcoRI*, are able to replicate. pRRI2 shows no significant similarity with the only other P. ruminicola plasmid to have been studied previously, pRAM4.
机译：pRRI2是来自瘤胃细菌小球藻Prevotella ruminicola 223 / M2 / 7的小型隐性质粒，已用于构建在许多拟杆菌/普氏杆菌菌株以及大肠杆菌中复制的穿梭载体（pRH3和pRRI207）。对pRRI2的序列分析表明，它是一个3240 bp的质粒，带有两个清晰的开放阅读框。由ORF1编码的代表。展示了与来自普通拟杆菌和脆弱拟杆菌的RepA蛋白的48％和47％的氨基酸序列同一性。分别。名为Pre的ORF2与来自Flavobacterium spp的推定质粒重组蛋白具有34％的氨基酸序列同一性。质粒pFL1和30％的氨基酸序列与脆弱型芽孢杆菌Tn5520的BmpH相同。用HindIII破坏ORF1可以防止拟杆菌属中的复制和维持。宿主，但携带pRRI2的穿梭载体在ORP2中中断。由EcoRI *提供，能够复制。 pRRI2与先前已研究过的唯一其他金黄色葡萄球菌质粒pRAM4没有显着相似性。

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