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首页> 外文期刊>Peptides: An International Journal >Orexin-A and ghrelin depolarize the same pedunculopontine tegmental neurons in rats: an in vitro study.
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Orexin-A and ghrelin depolarize the same pedunculopontine tegmental neurons in rats: an in vitro study.

机译：Orexin-A和Ghrelin使大鼠中相同的足小腿被膜神经元去极化：一项体外研究。

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摘要

Orexin (ORX), also called hypocretin, and ghrelin are newly identified peptides in the brain and/or peripheral organs, and they are involved in the regulation of sleep-wakefulness as well as feeding. In our previous studies we have found that ORX and ghrelin each depolarizes more than half of the cholinergic neurons recorded in the pedunculopontine tegmental nucleus (PPT) via a dual ionic mechanism including a decrease of K(+) conductance and an increase of nonselective cationic conductance. Thus, the present study was carried out to investigate whether ORX-A and ghrelin both depolarize the same PPT neuron. About 60% of PPT neurons examined was depolarized by both ORX-A and ghrelin, 20% by ORX-A alone, and 10% by ghrelin alone. The remaining 10% did not respond to these peptides. In neurons which were responsive to both ORX-A and ghrelin, the depolarizations induced by ORX-A and ghrelin were additive. In addition, the ORX-A- and ghrelin-induced depolarizations were both blocked by D609, a phosphatidylcholine-specific phospholipase C (PLC) inhibitor. These results suggest that same PPT neurons with receptors for ORX and ghrelin are involved in the cellular process through which ORX and ghrelin participate in the regulation of sleep wakefulness, and that the excitatory effects of ORX and ghrelin on PPT neurons are mediated by PLC.
机译：食欲肽（ORX）（也称为降血钙素）和生长素释放肽是脑和/或外周器官中新近鉴定出的肽，它们参与睡眠觉醒和进食的调节。在我们以前的研究中，我们发现ORX和Ghrelin各自通过双离子机制使K（+）电导减少和非选择性阳离子电导增加的双离子作用，使在足小脑桥被盖核（PPT）中记录的胆碱能神经元中的一半以上去极化。 。因此，进行本研究以调查ORX-A和ghrelin是否都使相同的PPT神经元去极化。大约60％的PPT神经元被ORX-A和ghrelin消极化，仅被ORX-A去除20％，仅被ghrelin去除10％。其余的10％对这些肽没有反应。在对ORX-A和Ghrelin都有反应的神经元中，由ORX-A和Ghrelin引起的去极化是累加的。此外，ORX-A和生长素释放肽诱导的去极化均被磷脂酰胆碱特异性磷脂酶C（PLC）抑制剂D609阻断。这些结果表明，具有ORX和ghrelin受体的相同PPT神经元参与了细胞过程，通过该过程，ORX和ghrelin参与了睡眠觉醒的调节，并且ORX和ghrelin对PPT神经元的兴奋作用是由PLC介导的。

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