• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proteomics >Proteomic investigation of Vibrio alginolyticus challenged Caenorhabditis elegans revealed regulation of cellular homeostasis proteins and their role in supporting innate immune system
【24h】

Proteomic investigation of Vibrio alginolyticus challenged Caenorhabditis elegans revealed regulation of cellular homeostasis proteins and their role in supporting innate immune system

机译：溶藻弧菌攻击秀丽隐杆线虫的蛋白质组学研究揭示了细胞稳态蛋白的调控及其在支持先天免疫系统中的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Caenorhabditis elegans has been the preferred model system for many investigators to study pathogenesis. In the present investigation, regulation of C. elegans proteome was explored against V. alginolyticus infection using quantitative proteomics approach. Proteins were separated using 2D-DIGE and the differentially regulated proteins were identified using PMF and MALDI TOF/TOF analysis. The results thus obtained were validated using Western blotting for candidate proteins. The corresponding transcriptional regulation was quantified subsequently using real-time PCR. Interaction network for candidate proteins was predicted using search tool for the retrieval of interacting genes/proteins (STRING) and functional validation was performed using respective mutant strains. Out of the 25 proteins identified, 21 proteins appeared to be upregulated while four were downregulated. Upregulated proteins included those involved in stress-response (PDI-2, HSP-6), immune-response (protein kinase -18, GST-8) and energy-production (ATP-2) while proteins involved in structural maintenance (IFB-2) and lipid metabolism (SODH-1) were downregulated. The roles of these players in the host system during Vibrio infection was analyzed in vivo using wild type and mutant C. elegans. Survival assays using mutants lacking pdi-2, ire-1, and xbp-1 displayed enhanced susceptibility to V. alginolyticus. Cellular stress generated by V. alginolyticus was determined using ROS assay. This is the first report of proteome changes in C. elegans against V. alginolyticus challenge and highlights the significance of unfolded protein response (UPR) pathway during bacterial infection.
机译：秀丽隐杆线虫已成为许多研究者研究发病机理的优选模型系统。在目前的调查中，探索线虫蛋白质组的调控，利用定量蛋白质组学方法对抗溶藻弧菌感染。使用2D-DIGE分离蛋白质，并使用PMF和MALDI TOF / TOF分析鉴定差异调节的蛋白质。使用蛋白质印迹法对候选蛋白质验证了如此获得的结果。随后使用实时PCR定量相应的转录调控。使用搜索工具预测相互作用蛋白/蛋白质的相互作用网络（STRING），预测候选蛋白的相互作用网络，并使用相应的突变菌株进行功能验证。在鉴定出的25种蛋白质中，有21种蛋白质似乎被上调，而四种则被下调。上调的蛋白质包括参与应激反应（PDI-2，HSP-6），免疫应答（蛋白质激酶-18，GST-8）和能量产生（ATP-2）的蛋白质，而参与结构维持的蛋白质（IFB- 2）和脂质代谢（SODH-1）被下调。使用野生型和突变秀丽隐杆线虫体内分析了这些参与者在弧菌感染期间在宿主系统中的作用。使用缺少pdi-2，ire-1和xbp-1的突变体进行的生存测定显示出对溶藻弧菌的敏感性增加。使用ROS测定法确定溶藻弧菌产生的细胞应激。这是线虫秀丽线虫抗溶藻弧菌攻击的蛋白质组变化的第一份报道，并突出了细菌感染过程中未折叠的蛋白质反应（UPR）途径的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号