Purification and characterization of the deoxynucleoside monophosphate kinase of bacteriophage T5

Mikoulinskaia GV.; Gubanov SI.; Zimin AA.; Kolesnikov IV.; Feofanov SA. Miroshnikov AI.

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Purification and characterization of the deoxynucleoside monophosphate kinase of bacteriophage T5

机译：T5噬菌体脱氧核苷单磷酸激酶的纯化与鉴定

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Deoxynucleoside monophosphate kinase (dNMP kinase) of bacteriophage T5 (EC 2.7.4.13) was purified to apparent homogeneity from phage-infected Escherichia coli cells. Electrophoresis in sodium dodecyl sulfate-polyacrylamide gel showed that the enzyme has a molecular mass of about 29 kDa. The molecular mass of dNMP kinase estimated by analytical equilibrium ultracentrifugation turned out to be 29.14 +/- 3.03 kDa. These data suggest that the enzyme exists in solution as a monomer. The isoelectric point of dNMP kinase was found to be 4.2. The N-terminal amino acid sequence, comprising 21 amino acids, was determined to be VLVGLHGEAGSGKDGVAKLII. A comparison of this amino acid sequence and those of known enzymes with a similar function suggests. the presence of a nucleotide-binding site in the sequenced region. (C) 2002 Elsevier Science (OSA). All rights reserved. [References: 30]

机译：从噬菌体感染的大肠杆菌细胞中纯化出噬菌体T5（EC 2.7.4.13）的脱氧核苷单磷酸激酶（dNMP激酶），使其具有明显的同质性。十二烷基硫酸钠-聚丙烯酰胺凝胶中的电泳表明该酶的分子量约为29 kDa。通过分析平衡超速离心法估计的dNMP激酶的分子量为29.14 +/- 3.03kDa。这些数据表明该酶以单体形式存在于溶液中。发现dNMP激酶的等电点为4.2。包含21个氨基酸的N端氨基酸序列被确定为VLVGLHGEAGSGKDGVAKLII。建议将该氨基酸序列与已知功能相似的酶进行比较。在测序区域中存在核苷酸结合位点。（C）2002 Elsevier Science（OSA）。版权所有。 [参考：30]

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《Protein Expression and Purification》 |2003年第2期|共7页
作者
Mikoulinskaia GV.; Gubanov SI.; Zimin AA.; Kolesnikov IV.; Feofanov SA. Miroshnikov AI.;
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正文语种 eng
中图分类蛋白质;
关键词
Ump-cmp kinase; Escherichia-coli; Protein; Expression; Sequence; Cloning; Complex; Enzyme; Phage; Genes;

机译：Ump-cmp激酶;大肠杆菌;蛋白质;表达;序列;克隆;复合;酶;噬菌体;基因;

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1. Purification and characterization of the deoxynucleoside monophosphate kinase of bacteriophage T5 [J] . Mikoulinskaia GV., Gubanov SI., Zimin AA., Protein Expression and Purification . 2003,第2期

机译：T5噬菌体脱氧核苷单磷酸激酶的纯化与鉴定
2. Isolation and characterization of a bacteriophage T5 mutant deficient in deoxynucleoside 5'-monophosphatase activity. [J] . T J Mozer, R B Thompson, S M Berget, Journal of Virology . 1977,第2期

机译：脱氧核苷5'-单磷酸酶活性的噬菌体T5突变体的分离与表征。
3. Isolation and characterization of a bacteriophage T5 mutant deficient in deoxynucleoside 5'-monophosphatase activity. [J] . T J Mozer, R B Thompson, S M Berget, Journal of Virology . 1977,第2期

机译：脱氧核苷5'-单磷酸酶活性的噬菌体T5突变体的分离与表征。
4. Characterization of Bacteriophage Derived Anti-staphylococcal Protein(P128) From Production to Purification Using Microfluidic based LC System Coupled to an Advanced QTOF-MS [C] . Ravindra Gudihal, Sundaram P.M, Madhavi H.N, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：使用微流体基于QTOF-MS的微流体基于QTOF-MS的微流体的LC系统来表征噬菌体衍生的抗葡萄球菌蛋白（P128）以纯化。
5. Expression, purification and characterization of bacteriophage lambda tail tip proteins. [D] . Dai, Xiaoxian. 2009

机译：噬菌体λ尾尖蛋白的表达，纯化和鉴定。
6. Isolation and Partial Characterization of Bacteriophage T5 Mutants Deficient in the Ability to Induce Deoxynucleoside Monophosphate Kinase [O] . Susan M. Berget, Huber R. Warner, D. James McCorquodale 1974

机译：噬菌体T5突变体缺乏诱导脱氧核苷单磷酸激酶能力的分离和部分表征。
7. Isolation and Partial Characterization of Bacteriophage T5 Mutants Deficient in the Ability to Induce Deoxynucleoside Monophosphate Kinase [O] . Susan M. Berget, Huber R. Warner, D. James McCorquodale 1974

机译：噬菌体T5突变体的分离和部分表征缺乏诱导脱氧核苷一磷酸氨基磷酶的能力

Purification and characterization of the deoxynucleoside monophosphate kinase of bacteriophage T5

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