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首页> 外文期刊>Protein Expression and Purification >Cloning and sequence analysis of the Korean strain of spike gene of porcine epidemic diarrhea virus and expression of its neutralizing epitope in plants
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Cloning and sequence analysis of the Korean strain of spike gene of porcine epidemic diarrhea virus and expression of its neutralizing epitope in plants

机译：猪流行性腹泻病毒穗基因韩国株的克隆，序列分析及中和表位在植物中的表达

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Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in pigs and leads to death with a high mortality rate, which has been reported notably in Korea. The spike (S) gene of the PEDV isolated in Korea was cloned and sequenced. The nucleotide sequence encoding the entire S gene open reading frame of Korean strain was 4161 bases long encoding 1387 amino acids. The neutralizing epitope of Korean PEDV (K-COE) was expressed in tobacco plants using Agrobacterium-mediated protein transformation. The recombinant K-COE constituted up to 0.1 % of the total soluble protein in the leaves of transgenic tobacco plants. The result of this study opens the way for the development of an edible vaccine against PEDV infection in Korea. (c) 2005 Elsevier Inc. All rights reserved.
机译：猪流行性腹泻病毒（PEDV）引起猪的急性腹泻和脱水，并导致高死亡率的死亡，这在韩国尤为明显。克隆并分离了韩国分离到的PEDV的穗（S）基因。编码韩国菌株的整个S基因开放阅读框的核苷酸序列为4161个碱基长，编码1387个氨基酸。使用农杆菌介导的蛋白质转化，在烟草植物中表达了韩国PEDV（K-COE）的中和表位。重组K-COE占转基因烟草植物叶片中总可溶性蛋白的0.1％。这项研究的结果为在韩国开发可预防PEDV感染的可食用疫苗开辟了道路。 （c）2005 Elsevier Inc.保留所有权利。

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