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首页> 外文期刊>Urology >Gene expression profiles in the Peyronie's disease plaque.
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Gene expression profiles in the Peyronie's disease plaque.

机译：佩罗尼氏菌斑中的基因表达谱。

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OBJECTIVES: To provide molecular insight into the pathophysiology of Peyronie's disease (PD), a preliminary profile of differential gene expression between the PD plaque and control tunica albuginea was obtained with DNA microarrays. METHODS: Seven PD plaques and five control tunica albugineas were studied. cDNA specimens were prepared from RNA isolated from one calcified PD plaque and one control tissue and hybridized with the Clontech Atlas 1.2 Array. Another set of plaque and control RNA samples was hybridized with the Affymetrix GeneChip. Relative changes of greater than 2.0 defined up-regulation and down-regulation, respectively. RNA from the remaining tissues was used to determine, by reverse transcriptase-polymerase chain reaction and Western blot analysis, the expression of selected individual genes. RESULTS: Some of up-regulated genes in the PD plaque detected by the Clontech assay were pleiotrophin, monocyte chemotactic protein 1, and early growth response protein, which are involved in osteoblast recruitment, inflammation, and fibroblast proliferation, respectively. Ubiquitin and Id-2, which are involved in tissue remodeling, were down-regulated. The Affymetrix DNA chips identified the up-regulation of elastase (involved in elastic fiber degradation) and the myofibroblast markers alpha and gamma-smooth muscle actin, desmin, and others, as well as the down-regulation of collagenase IV and transforming growth factor-beta modulators. Four of the five genes selected for reverse transcriptase-polymerase chain reaction and Western blotting confirmed the DNA microarray results. CONCLUSIONS: In the PD tissue, the genes involved in collagen synthesis, myofibroblast differentiation, tissue remodeling, inflammation, ossification, and proteolysis are up-regulated, and the genes that inhibit some of these processes and collagenase are down-regulated.
机译：目的：为了提供分子生物学对佩罗尼氏病（PD）的病理生理学的了解，利用DNA微阵列初步获得了PD斑块和白膜控制性膜之间差异基因表达的初步概况。方法：研究了7个PD斑块和5个对照白膜。从从一种钙化的PD斑块和一种对照组织中分离的RNA制备cDNA标本，并与Clontech Atlas 1.2 Array杂交。将另一组噬菌斑和对照RNA样品与Affymetrix GeneChip杂交。大于2.0的相对变化分别定义了上调和下调。通过逆转录酶-聚合酶链反应和蛋白质印迹分析，将来自剩余组织的RNA用于确定所选单个基因的表达。结果：通过Clontech分析检测到的PD斑块中的一些上调基因是多效蛋白，单核细胞趋化蛋白1和早期生长反应蛋白，它们分别与成骨细胞募集，炎症和成纤维细胞增殖有关。参与组织重塑的泛素和Id-2被下调。 Affymetrix DNA芯片识别出了弹性蛋白酶的上调（涉及弹性纤维降解）以及成肌纤维细胞标志物α和γ平滑肌肌动蛋白，结蛋白等，以及胶原酶IV和转化生长因子的下调。 β调节剂。选择用于逆转录聚合酶链反应和蛋白质印迹的五个基因中的四个证实了DNA芯片结果。结论：在PD组织中，参与胶原合成，成肌纤维细胞分化，组织重塑，炎症，骨化和蛋白水解的基因被上调，而抑制这些过程和胶原酶的基因被下调。

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