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首页> 外文期刊>Talanta: The International Journal of Pure and Applied Analytical Chemistry >Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles
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Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles

机译：钌配合物共价键合二氧化硅纳米粒子的制备及其时控发光生物成像应用

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摘要

Luminescent ruthenium(II) complex covalently bound silica nanoparticles have been prepared and used as a probe for time-gated luminescence bioimaging. The new nanoparticles were prepared by copolymerization of a luminescent Ru(II) complex tris(5-amino-1,10-phenanthroline)ruthenium(II) conjugated with 3-aminopropyl(triethoxy)si lane (APS-Ru conjugate), free (3 -aminopropyl)triethoxysilane (APS) and tetraethyl orthosilicate (TEOS) in a water-in-oil reverse microemulsion consisting of Triton X-100, n-octanol, cyclohexane and water in the presence of aqueous ammonia. Characterization by transmission electron microscopy indicates that the nanoparticles are monodisperse, spherical and uniform in size, 64 +/- 4 nm in diameter. Compared with the dye-doping nanoparticles, dye leakage of the new nanoparticles was remarkably decreased. In addition, it was found that the Ru(II) complex luminescence could be effectively enhanced with a longer luminescence lifetime (similar to 2.3 mu s) after forming the nanoparticles, which enables the nanoparticles to be suitable as a bioprobe for time-gated luminescence bioimaging applications. The nanoparticle-labeled streptavidin was prepared and successfully used for time-gated luminescence imaging detection of an environmental pathogen, Giardia lamblia, with high specificity and sensitivity.
机译：已经制备了发光钌（II）配合物共价键合的二氧化硅纳米粒子，并将其用作时间门控发光生物成像的探针。新的纳米粒子通过共轭发光的Ru（II）配合物与（3-APS-Ru共轭）3-氨基丙基（三乙氧基）si通道的三（5-氨基-1,10-菲咯啉）钌（II）共聚制备。在氨水的存在下，由Triton X-100，正辛醇，环己烷和水组成的油包水反向微乳液中的3-氨基丙基）三乙氧基硅烷（APS）和原硅酸四乙酯（TEOS）。通过透射电子显微镜表征表明纳米颗粒是单分散的，球形的并且尺寸均匀，直径为64 +/- 4nm。与掺杂纳米粒子相比，新型纳米粒子的染料泄漏显着减少。另外，发现形成纳米颗粒后，Ru（II）配合物发光可以以更长的发光寿命（类似于2.3μs）有效地增强，这使得纳米颗粒适合作为时间门控发光的生物探针。生物成像应用。制备了纳米级标记的抗生蛋白链菌素，并以高特异性和高灵敏度成功地用于环境病原体贾第鞭毛虫的时间门控发光成像检测。

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