...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pathology Research and Practice >Synthetic (glyco-)peptides of the homophilic recognition domain of E-cadherin lead to increased E-cadherin mRNA synthesis and are inductors of cell differentiation in primary lung cancer cell lines.
【24h】

Synthetic (glyco-)peptides of the homophilic recognition domain of E-cadherin lead to increased E-cadherin mRNA synthesis and are inductors of cell differentiation in primary lung cancer cell lines.

机译：E-钙粘蛋白的同源识别结构域的合成（糖）肽导致E-钙粘蛋白mRNA合成增加，并且是原发性肺癌细胞系中细胞分化的诱导剂。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

E-cadherin is one of the critical molecules involved in the metastatic process in many types of cancer. Once combined, E-cadherin exceeds the amount of membranous E-cadherin on the cellular surface by activation of intracellular signaling cascades. Studies on transformed keratinocytes of the HaCat cell line showed induction of differentiation by synthetical partial structures of the homophilic binding region of E-cadherin. The knowledge of effects in lung cancer cells is sparse. Therefore, the effects in primary lung cancer cell lines were investigated. Four primary lung cancer cell lines were incubated for 3, 6, 12, 15, 18, and 24h with synthetic partial structures (peptide and glycopeptide). The control substance was sodium butyrate. mRNA was isolated, and relative quantification of E-cadherin was performed using the Real-Time PCR. During the stimulation period, morphologic pictures were taken, and immunohistochemical staining of membranous E-cadherin was performed. Life/dead assays were used to display cell vitality. The intracellular E-cadherin mRNA amount was increased after incubation with the synthetic partial structures. Life/dead assays showed improved survival and integrated cell/cell bindings after stimulation with the partial structures. Increased cell mortality was revealed after sodium butyrate incubation. An effect mediated via E-cadherin on the cellular surface is proposed. The two synthetic partial structures of the homophilic binding region of E-cadherin increased the intracellular E-cadherin mRNA amount, cell-cell bindings, and survival of the tumor cells. Extracellular binding by synthetic partial structures to the binding region may have a beneficial influence on tumor progression in the metastatic process.
机译：E-钙黏着蛋白是许多类型的癌症转移过程中涉及的关键分子之一。一旦结合，E-钙粘着蛋白通过激活细胞内信号传导级联而超过细胞表面上的膜状E-钙粘着蛋白的量。 HaCat细胞系转化的角质形成细胞的研究表明，通过E-钙粘着蛋白的同源结合区的合成部分结构诱导分化。在肺癌细胞中作用的知识很少。因此，研究了在原代肺癌细胞系中的作用。将四个原代肺癌细胞系与合成的部分结构（肽和糖肽）一起孵育3、6、12、15、18和24h。对照物质是丁酸钠。分离mRNA，并使用实时PCR进行E-钙粘蛋白的相对定量。在刺激期间，拍摄形态照片，并进行膜E-钙粘蛋白的免疫组织化学染色。生命/死亡测定用于显示细胞活力。与合成的部分结构孵育后，细胞内E-钙粘着蛋白mRNA的量增加。生命/死亡分析显示，在用部分结构刺激后，存活率提高，并且细胞/细胞结合整合。丁酸钠孵育后发现细胞死亡率增加。提出了通过E-钙粘蛋白介导的在细胞表面上的作用。 E-钙粘着蛋白的同源结合区域的两个合成的部分结构增加了细胞内E-钙粘着蛋白mRNA的量，细胞间结合以及肿瘤细胞的存活。通过合成的部分结构与结合区域的细胞外结合可能对转移过程中的肿瘤进展产生有益的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号