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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Mature Erythrocyte Surface Antigen Protein Identified in the Serum of Plasmodium falciparum-Infected Patients
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Mature Erythrocyte Surface Antigen Protein Identified in the Serum of Plasmodium falciparum-Infected Patients

机译：在恶性疟原虫感染的患者血清中鉴定出成熟的红细胞表面抗原蛋白

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摘要

This study was performed to identify circulating Plasmodium falciparum proteins in patient serum, which may be useful as diagnostic markers. Depletion of highly abundant proteins from each pooled serum sample obtained from P falciparum-infected patients and healthy individuals was performed using the Proteoseek Antibody-Based Albumin/IgG Removal Kit (Thermo Scientific, Rockford, IL). In analysis 1, the depleted serum was analyzed directly by NanoLC-MS/MS. In analysis 2, the depleted serum was separated by two-dimensional electrophoresis followed by western blot analysis. Subsequently, the selected band was analyzed by NanoLC-MS/MS. The result of analysis 1 revealed the presence of two mature erythrocyte surface antigen (MESA) proteins and chloroquine resistance transporter protein (PfCRT). In addition, analysis 2 revealed an antigenic 75-kDa band when the membrane was probed with purified IgG from the pooled serum obtained from P falciparum-infected patients. MS/MS analysis of this protein band revealed fragments of P falciparum MESA proteins. Thus, in this study, two different analyses revealed the presence of Plasmodium MESA protein in pooled serum from malaria patients; thus, this protein should be further investigated to determine its usefulness as a diagnostic marker.
机译：进行该研究以鉴定患者血清中循环的恶性疟原虫蛋白质，其可用作诊断标记。使用基于Proteoseek抗体的白蛋白/ IgG去除试剂盒（Thermo Scientific，Rockford，IL），从恶性疟原虫感染的患者和健康个体的每个合并血清样品中消耗高度丰富的蛋白质。在分析1中，通过NanoLC-MS / MS直接分析了耗尽的血清。在分析2中，通过二维电泳分离消耗的血清，然后进行蛋白质印迹分析。随后，通过NanoLC-MS / MS分析所选波段。分析1的结果表明存在两种成熟的红细胞表面抗原（MESA）蛋白和氯喹抗性转运蛋白（PfCRT）。此外，分析2显示，当用纯化的IgG从恶性疟原虫感染患者的合并血清中纯化的IgG探测膜时，发现一条75 kDa的抗原性条带。对该蛋白条带的MS / MS分析揭示了恶性疟原虫MESA蛋白的片段。因此，在这项研究中，两项不同的分析揭示了疟疾患者合并血清中存在疟原虫MESA蛋白。因此，应进一步研究该蛋白以确定其作为诊断标志物的有用性。

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