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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Survival and growth of Shigella flexneri, Salmonella enterica serovar enteritidis, and Vibrio cholerae O1 in reconstituted infant formula.
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Survival and growth of Shigella flexneri, Salmonella enterica serovar enteritidis, and Vibrio cholerae O1 in reconstituted infant formula.

机译：重组婴儿配方奶粉中志贺氏菌，肠炎沙门氏菌，肠炎沙门氏菌和霍乱弧菌O1的存活和生长。

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Formula feeding is an alternative method to prevent mother-to-child infection with human immunodeficiency virus through breast-feeding in developing countries. Growth of bacterial pathogens in reconstituted infant formula has become a health hazard when contaminated water is used for rehydration. This study was conducted to assess bacterial safety risk of using contaminated water to reconstitute infant formula. Survival and growth characteristics were determined for three bacterial pathogens, Vibrio cholerae O1, Shigella flexneri, and Salmonella enterica serovar Enteritidis, inoculated into sterile tap water (3.2-3.4 log10 colony-forming units [CFU]/ml) and infant formula (1.5-1.7 and 3.2-3.4 log10 CFU/ml) and incubated at 4 degrees C or 30 degrees C for up to 24 hours. Vibrio cholerae O1 was the most sensitive of the three pathogens when inoculated into water, with no viable cells detected within 2 hours at 4 degrees C or 30 degrees C. The rate of inactivation in water was greater at 30 degrees C than at 4 degrees C. Vibrio cholerae O1, Shigella flexneri, and Salmonella enterica serovar Enteritidis grew rapidly in infant formula at 30 degrees C, reaching populations of 9.2, 8.7, and 9.2 log10 CFU/ml, respectively, at 24 hours. Populations of all three pathogens did not change significantly after incubating infant formula for 24 hours at 4 degrees C, but continuously decreased in water throughout incubation for 24 hours, regardless of temperature. Results suggest that unless refrigerated, reconstituted infant formula should be consumed soon after preparation to avoid increased risk of illness associated with increases in populations of pathogenic bacteria that may be introduced by contaminated water.
机译：配方奶喂养是在发展中国家通过母乳喂养预防母婴感染人类免疫缺陷病毒的另一种方法。当使用被污染的水补液时，重构婴儿配方食品中细菌病原体的生长已危害健康。这项研究旨在评估使用受污染的水配制婴儿配方食品的细菌安全性风险。确定了三种细菌性病原体的存活和生长特征，分别是霍乱弧菌O1，霍乱志贺氏菌和肠炎沙门氏菌肠炎沙门氏菌，并接种到无菌自来水中（3.2-3.4 log10 log10菌落形成单位[CFU] / ml）和婴儿配方奶粉（1.5- 1.7和3.2-3.4 log10 CFU / ml），并在4摄氏度或30摄氏度下孵育长达24小时。霍乱弧菌O1接种到水中后是三种病原体中最敏感的，在4摄氏度或30摄氏度的2小时内未检测到活细胞。在30摄氏度下，水中的灭活速率大于在4摄氏度下。霍乱弧菌O1，志贺氏志贺氏菌和肠炎沙门氏菌肠炎沙门氏菌在30摄氏度的婴儿配方食品中快速生长，在24小时时分别达到9.2、8.7和9.2 log10 CFU / ml的种群。在4摄氏度下将婴儿配方奶粉孵育24小时后，所有三种病原体的种群都没有显着变化，但是无论温度如何，在整个孵育24小时后水中的水量持续减少。结果表明，除非冷藏，否则重组后的婴儿配方食品应在制备后立即食用，以避免因污染水引起的致病菌数量增加而增加患病的风险。

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