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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >A simple fluorogenic method to detect Vibrio cholerae and Aeromonas hydrophila in well water for areas impacted by catastrophic disasters.
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A simple fluorogenic method to detect Vibrio cholerae and Aeromonas hydrophila in well water for areas impacted by catastrophic disasters.

机译：一种简单的荧光检测方法，可在受灾难性灾难影响的地区检测井水中的霍乱弧菌和嗜水气单胞菌。

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摘要

The colony overlay procedure for peptidases (COPP) is a simple, fluorogenic assay that can rapidly detect and quantify Vibrio cholerae and Aeromonas hydrophila in well water. Cleavage of the substrate L-lysyl-7-amino-4-trifluoromethylcoumarin by enzymes present in Vibrio and Aeromonas species produces fluorescent foci on cellulose acetate membranes exposed to long-wave ultraviolet light. Vibrio cholerae O1, O139, O155, and A. hydrophila were readily detected using this procedure, whereas Enterobacteriaceae and other non-Vibrionaceae pathogens did not produce fluorescence. The assay is practical for assessing the relative safety of well water in areas that have experienced catastrophic devastation from natural disasters, acts of war, or civil strife and may help curb outbreaks of cholera and other enteric illnesses in affected areas. In tropical climates, the procedure may be adapted for use in areas without electricity.
机译：肽酶的菌落覆盖程序（COPP）是一种简单的荧光检测方法，可以快速检测和定量井水中的霍乱弧菌和亲水性嗜水气单胞菌。弧菌和气单胞菌属物种中存在的酶对底物L-赖氨酰基-7-氨基-4-三氟甲基香豆素的切割会在暴露于长波紫外线的醋酸纤维素膜上产生荧光灶。霍乱弧菌O1，O139，O155和亲水曲霉很容易使用此程序进行检测，而肠杆菌科和其他非弧菌科病原体则不会产生荧光。该方法可用于评估遭受自然灾害，战争或内乱的灾难性破坏的地区的井水的相对安全性，并可能有助于遏制霍乱和其他肠道疾病在受影响地区的爆发。在热带气候下，该程序可能适合在没有电的地区使用。

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