Recovery and use of Plasmodium DNA from malaria rapid diagnostic tests.

Veron V; Carme B

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Recovery and use of Plasmodium DNA from malaria rapid diagnostic tests.

机译：从疟疾快速诊断测试中回收和使用疟原虫DNA。

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The purpose of this study was to show that individual malaria rapid diagnosis tests (MRDTs) could also be used to isolate Plasmodium DNA for genetic studies. We extracted and amplified Plasmodium DNA using two commercial MRDT kits. Phenol/chloroform extraction followed by a nested polymerse chain reaction (PCR) can be used to identify Plasmodium falciparum and Plasmodium vivax from MRDTs. The PCR on MRDT-isolated DNA was more sensitive than antigen capture by MRDT. Satisfactory results were also obtained if older MRDT tests were used, even after long periods of storage at ambient temperature, with no special preservation.

机译：这项研究的目的是表明，个体疟疾快速诊断测试（MRDT）也可用于分离疟原虫DNA进行遗传研究。我们使用两种商业MRDT试剂盒提取并扩增了疟原虫DNA。苯酚/氯仿提取，然后进行巢状聚合物链反应（PCR），可用于从MRDT中鉴定恶性疟原虫和间日疟原虫。 MRDT分离的DNA上的PCR比MRDT捕获抗原更敏感。如果使用较旧的MRDT测试，即使在环境温度下长时间存储，也没有特殊保存，也会获得令人满意的结果。

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来源
《The American Journal of Tropical Medicine and Hygiene》 |2006年第6期|共3页
作者
Veron V; Carme B;
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正文语种 eng
中图分类预防医学、卫生学;
关键词
DNA; Plasmodium; Malaria; Rapid; recovery from disease; 疟原虫属; 疟疾;

机译：DNA;Plasmodium;Malaria;Rapid;recovery from disease;疟原虫属;疟疾;

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1. Recovery and use of Plasmodium DNA from malaria rapid diagnostic tests. [J] . Veron V, Carme B The American Journal of Tropical Medicine and Hygiene . 2006,第6期

机译：从疟疾快速诊断测试中回收和使用疟原虫DNA。
2. The heat stability of Plasmodium lactate dehydrogenase-based and histidine-rich protein 2-based malaria rapid diagnostic tests. [J] . Chiodini PL, Bowers K, Jorgensen P, Transactions of the Royal Society of Tropical Medicine and Hygiene . 2007,第4期

机译：基于乳酸疟原虫脱氢酶和富含组氨酸的蛋白质2的疟疾的热稳定性快速诊断测试。
3. Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria -assessment of DNA extraction methods and field applicability [J] . Ulrika Morris, Berit Aydin-Schmidt, Delér Shakely, Malaria Journal . 2013,第1期

机译：疟原虫疟原虫疟原虫疟疾分子监测的快速诊断试验 - DNA提取方法和现场适用性
4. Development of Dried Plasmodium falciparum Samples for Quality Control of Malaria rapid diagnostics tests and as proficiency testing panels [C] . M. Aidoo Annual Meeting of the American College of Veterinary Pathologists . 2015

机译：疟疾疟原虫疟原虫样品的开发快速诊断检验及熟练试验面板
5. The Effectiveness of Home Based Management of Uncomplicated Malaria Using Artemisinin Combination Treatments (ACTs) and Rapid Diagnostic Tests (RDTs) in Rural Senegal (West Africa): Pilot Study in Three Districts. [D] . Seck, Ibrahima. 2017

机译：塞内加尔农村地区（西非）使用青蒿素联合治疗（ACT）和快速诊断测试（RDT）对家庭进行简单疟疾管理的有效性：三个地区的初步研究。
6. Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria -assessment of DNA extraction methods and field applicability [O] . Ulrika Morris, Berit Aydin-Schmidt, Delér Shakely, 2013

机译：恶性疟原虫疟疾分子监测的快速诊断测试-DNA提取方法的评估和现场适用性
7. Bead-based immunoassay allows sub-picogram detection of histidine-rich protein 2 from Plasmodium falciparum and estimates reliability of malaria rapid diagnostic tests. [O] . Eric Rogier, Mateusz Plucinski, Naomi Lucchi, 2017

机译：基于珠子的免疫分析允许从恶性疟原虫的富含组氨酸的蛋白质2进行亚皮克检测，并估计疟疾快速诊断测试的可靠性。

Recovery and use of Plasmodium DNA from malaria rapid diagnostic tests.

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