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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Amniotic fluid is not useful for diagnosis of congenital Trypanosoma cruzi infection.
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Amniotic fluid is not useful for diagnosis of congenital Trypanosoma cruzi infection.

机译：羊水不能用于诊断先天性克氏锥虫感染。

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摘要

Although Trypanosoma cruzi can be transmitted transplacentally and induce congenital infection, no data are available about the presence of this parasite in human amniotic fluid. We examined 8, 19, and 4 amniotic fluid samples (collected at delivery or by aspiration of gastric content of neonates) from control uninfected mothers (M-B-), infected mothers delivering uninfected newborns (M+B-), and mothers of confirmed congenital cases (M+B+), respectively. Polymerase chain reaction (PCR), using nuclear and kinetoplastic DNA primers (Tcz1-Tcz2 and 121-122), were negative for all control M-B- samples, but positive for 5 of 19 M+B- and 2 of 4 M+B+ samples. To determine the number of parasites in the positive samples, real-time PCR using S35/S36 kinetoplastic DNA was performed. Only one M+B+ sample presented a high parasitic DNA amount, whereas the other six PCR-positive samples displayed traces of T. cruzi DNA. In conclusion, the release of parasites in amniotic fluid is probably a rare event that cannot behelpful for the routine diagnosis of congenital Chagas disease.
机译：尽管克氏锥虫可以经胎盘传播并诱发先天性感染，但尚无有关人羊水中存在该寄生虫的数据。我们检查了8例，19例和4例羊水样本（在分娩时或通过吸入新生儿的胃内物收集），分别来自未感染的对照母亲（MB-），未感染的新生儿（M + B-）和确诊的先天性母亲情况（M + B +）。使用核和动塑料DNA引物（Tcz1-Tcz2和121-122）的聚合酶链反应（PCR）对所有对照MB-样品均为阴性，但对19 M + B-的5个和4 M + B +的2个为阳性。为了确定阳性样品中的寄生虫数量，使用S35 / S36运动塑料DNA进行了实时PCR。只有一个M + B +样品表现出较高的寄生DNA量，而其他六个PCR阳性样品则显示出曲霉T. cruzi DNA的痕迹。总之，羊水中寄生虫的释放可能是罕见事件，对常规诊断先天性南美锥虫病无济于事。

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