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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Genetic complexity of Plasmodium falciparum gametocytes isolated from the peripheral blood of treated Gambian children.
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Genetic complexity of Plasmodium falciparum gametocytes isolated from the peripheral blood of treated Gambian children.

机译：从治疗的冈比亚儿童的外周血中分离出的恶性疟原虫配子细胞的遗传复杂性。

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摘要

The genetic complexity of Plasmodium falciparum gametocytes isolated from Gambian children participating in a controlled trial of anti-malarial therapy was investigated. RNA and DNA were prepared from gametocyte-positive blood, which was also used in transmission experiments with Anopheles gambiae mosquitoes. Amplification by a reverse transcriptase-polymerase chain reaction (RT-PCR) of transcripts from the genes for the ring-infected erythrocyte surface antigen and the 16-kD antigen, which exhibit asexual and sexual stage-specific expression, was used to identify 30 post-treatment gametocyte isolates in which trophozoites persisted below the threshold of detection by microscopy. These included isolates from children who received sulfadoxine/pyrimethamine plus artesunate. Twenty-nine gametocyte-positive isolates that were free of subpatent trophozoites were examined further by PCR amplification of polymorphic genomic loci. We estimate that an average minimum of 2.3 genotypes occurred in these gametocyte-only isolates, and many of these were shown to be infective to mosquitoes. Thus, meiotic recombination between different genotypes is predicted to be a common event in this study area.
机译：研究了从参加抗疟疾治疗对照试验的冈比亚儿童中分离出的恶性疟原虫配子细胞的遗传复杂性。 RNA和DNA是从配子体阳性血液中制备的，也可用于冈比亚按蚊的传播实验。通过逆转录酶-聚合酶链反应（RT-PCR）扩增环感染的红细胞表面抗原和16-kD抗原基因的转录本，这些基因表现出无性和有性阶段特异性表达，用于鉴定30处理的配子分离物，其中滋养体持续低于显微镜检测的阈值。其中包括接受磺胺多辛/乙胺嘧啶加青蒿琥酯的儿童分离株。通过多态性基因组基因座的PCR扩增进一步检查了29个不含亚营养体的配子体阳性分离株。我们估计，在这些仅配子体分离物中，平均至少出现了2.3种基因型，其中许多基因型对蚊子具有感染性。因此，预计不同基因型之间的减数分裂重组是该研究领域的常见事件。

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