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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Streptococcus suis II immunoassay based on thorny gold nanoparticles and surface enhanced Raman scattering
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Streptococcus suis II immunoassay based on thorny gold nanoparticles and surface enhanced Raman scattering

机译：基于棘手的金纳米颗粒和表面增强拉曼散射的猪链球菌Ⅱ免疫分析

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摘要

An immunoassay based on surface enhanced Raman scattering (SERS) spectroscopy was developed to detect muramidase released protein (MRP) antibody against Streptococcus suis II (SS2) utilizing thorny gold nanoparticles (tAuNPs) as SERS substrates. Initially, tAuNPs with multi-branches were prepared by the seed-mediated growth method in the absence of templates and surfactants, facilitating p-mercaptobenzoic acid (pMBA) conjugation covalently onto the tAuNPs through S-Au bonds. The obtained immuno-SERS tag affording strong Raman signals made it possible to establish an application of indirect detection of the MRP antibody against SS2 with a sandwich assay at a highly sensitive level. The Raman intensity at 1588 cm ~(-1) was proportional to the logarithm of the concentration of MRP antibody in the range of 10 pg mL ~(-1) to 0.1 μg mL ~(-1). The detection sensitivity was significantly improved to 0.1 pg mL ~(-1) by using the immuno-SERS tags. Furthermore, the proposed SERS approach was applied to detect MRP antibody in pig serum samples, and the results agreed well with those of ELISA, indicating great potential for clinical application in diagnostic immunoassays. This journal is
机译：开发了一种基于表面增强拉曼散射（SERS）光谱的免疫分析方法，以棘手的金纳米颗粒（tAuNPs）作为SERS底物来检测抗猪链球菌II（SS2）的muramidase释放蛋白（MRP）抗体。最初，在没有模板和表面活性剂的情况下，通过种子介导的生长方法制备了具有多分支的tAuNP，从而促进p-巯基苯甲酸（pMBA）通过S-Au键共价结合到tAuNP上。所获得的提供强拉曼信号的免疫SERS标签使得建立高灵敏度水平的三明治检测间接检测针对SS2的MRP抗体成为可能。 1588 cm〜（-1）处的拉曼强度与MRP抗体浓度的对数成正比，范围为10 pg mL〜（-1）至0.1μgmL〜（-1）。通过使用免疫SERS标签，检测灵敏度显着提高至0.1 pg mL〜（-1）。此外，所提出的SERS方法被用于检测猪血清样品中的MRP抗体，其结果与ELISA法相吻合，表明其在诊断性免疫测定中的临床应用潜力很大。这本日记是

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