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首页> 外文期刊>The Biochemical Journal >Triticum aestivum L. endoxylanase inhibitor (TAXI) consists of two inhibitors, TAXI I and TAXI II, with different specificities.
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Triticum aestivum L. endoxylanase inhibitor (TAXI) consists of two inhibitors, TAXI I and TAXI II, with different specificities.

机译：普通小麦内切木聚糖酶抑制剂（TAXI）由两种具有不同特异性的抑制剂TAXI I和TAXI II组成。

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摘要

The Triticum aestivum L. endoxylanase inhibitor (TAXI) discovered by Debyser and Delcour [(1997) Eur. Pat. filed April 1997, published as WO 98/49278] and Debyser, Derdelinckx and Delcour [(1997) J. Am. Soc. Brew. Chem. 55, 153-156] seems to be a mixture of two different endoxylanase inhibitors, called TAXI I and TAXI II. By using Aspergillus niger as well as Bacillus subtilis endoxylanases for assaying inhibition activity, both inhibitors could be purified to homogeneity from wheat (Triticum aestivum L., var. Soissons). TAXI I and TAXI II have similar molecular structures. They both have a molecular mass of approx. 40.0 kDa, are not glycosylated and occur in two molecular forms, i.e. a non-proteolytically processed one and a proteolytically processed one. However, the pI of TAXI II (at least 9.3) is higher than that of TAXI I (8.8). TAXI I and TAXI II clearly show different inhibition activities towards different endoxylanases. The N-terminal amino acid sequences of both inhibitors show a high degree of identity, which might indicate that there is an evolutionary relationship between them.
机译：Debyser和Delcour [（1997）Eur。拍。 1997年4月提交，公开为WO 98/49278]和Debyser，Derdelinckx和Delcour [（1997）J. Am。 Soc。酿造。化学55，153-156]似乎是两种不同的木聚糖内切酶抑制剂的混合物，称为TAXI I和TAXI II。通过使用黑曲霉以及枯草芽孢杆菌内切木聚糖酶来测定抑制活性，可以将两种抑制剂从小麦中纯化至均质（Triticum aestivum L.，var.Soissons）。 TAXI I和TAXI II具有相似的分子结构。它们两者的分子质量均为约。 40.0kDa未被糖基化并且以两种分子形式存在，即，非蛋白水解处理的一种和蛋白水解处理的一种。但是，TAXI II的pI（至少9.3）高于TAXI I的pI（8.8）。 TAXI I和TAXI II清楚地显示出对不同内切木聚糖酶的不同抑制活性。两种抑制剂的N末端氨基酸序列都具有高度同一性，这可能表明它们之间存在进化关系。

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