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首页> 外文期刊>The European Journal of Neuroscience >Small cardioactive peptide gene: structure, expression and mass spectrometric analysis reveals a complex pattern of co-transmitters in a snail feeding neuron.
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Small cardioactive peptide gene: structure, expression and mass spectrometric analysis reveals a complex pattern of co-transmitters in a snail feeding neuron.

机译：小心脏活性肽基因：结构，表达和质谱分析揭示了蜗牛饲养神经元中辅助递质的复杂模式。

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摘要

The small cardioactive peptides (SCPs) are an important group of neural cotransmitters in molluscs where they are known to play both central and peripheral modulatory roles in the control of feeding behaviour. Here we show that in the snail Lymnaea the SCP gene exists in one interrupted copy that produces a single species of transcript which encodes a prepropeptide containing two structurally related SCPs SGYLAFPRMamide (SCP(A)) and pQNYLAFPRMamide (SCP(B)). In situ hybridization was used to localize expression specifically to the soma of several types of motoneurons in the feeding system of Lymnaea, including the giant B2 foregut motoneurons. The peptide content of individual B2 cell bodies was analysed by matrix-assisted laser desorption/ionization mass spectrometry and the structures of the SCPs predicted from the cloned gene were confirmed in these cells by post-source decay fragmentation analysis. Identical stimulatory activity for the two SCP peptides was demonstrated by their application to the isolated foregut, suggesting that their co-release from the B2 cells may play an important part in the co-modulation of gut motility, together with acetylcholine and the myomodulin family of peptides.
机译：小型心脏活性肽（SCPs）是软体动物中重要的一组神经共递质，其中它们在控制喂养行为中起着中枢和外周调节作用。在这里，我们显示出在蜗牛的Lymnaea中，SCP基因存在一个中断的拷贝中，该拷贝产生一个单一的转录本物种，该转录本编码包含两个结构相关的SCP SGYLAFPRMamide（SCP（A））和pQNYLAFPRMamide（SCP（B））的前肽。原位杂交用于将表达专门定位于Lymnaea饲养系统中几种类型的运动神经元的体细胞，包括巨型B2前肠运动神经元。通过基质辅助激光解吸/电离质谱分析了单个B2细胞体的肽含量，并通过源后衰变片段分析证实了从克隆基因预测的SCP的结构。这两种SCP肽对分离的前肠的应用证明了它们具有相同的刺激活性，表明它们从B2细胞的共释放可能与乙酰胆碱和肌调制蛋白家族一起在肠道运动的共调节中起重要作用。肽。

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