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首页> 外文期刊>The European Journal of Neuroscience >D2-like dopamine receptor activation excites rat dorsal raphe 5-HT neurons in vitro.
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D2-like dopamine receptor activation excites rat dorsal raphe 5-HT neurons in vitro.

机译：D2样多巴胺受体激活在体外激发大鼠背缝5-HT神经元。

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摘要

The aim of the present study was to investigate the effect of dopamine (DA) on the excitability of dorsal raphe nucleus (DRN) 5-hydroxytryptamine (5-HT) neurons using the patch-clamp technique in brain slices. Bath application of DA (1-300 microM) produced a concentration-dependent membrane depolarization in all 5-HT neurons examined. This effect persisted in the presence of tetrodotoxin (TTX; 1 microM) and low extracellular calcium. Moreover, blockade of ionotropic glutamate receptors with 6,7-dinitroquinoxaline-2,3-dione (DNQX) and 2-amino-5-phosphonopentanoic acid (AP5) did not prevent DA-induced depolarization, indicating that it was mediated by a direct effect of DA on 5-HT neurons. The DA-induced depolarization was not antagonized by selective alpha1-adrenergic receptor antagonists, prazosin and WB 4101, but by a nonselective DA receptor antagonist, haloperidol. In addition, the selective D2-like receptor agonist quinpirole and antagonist sulpiride mimicked and blocked DA-induced depolarization, respectively. These results indicate that DA-induced membrane depolarization in DRN 5-HT neurons is mediated by the activation of D2-like DA receptors. The DA-induced membrane depolarization and inward current were associated with an increase in membrane conductance. Examination of the current-voltage (I-V) relationship for the DA-induced inward current revealed that the amplitude of the current increased with membrane hyperpolarization and reversed polarity at a potential near -15 mV. These data suggest that DA-induced depolarization in DRN 5-HT neurons is not mediated by a decrease in potassium conductance, but most likely by the activation of a nonselective cation current.
机译：本研究的目的是使用膜片钳技术在脑片中研究多巴胺（DA）对背沟核（DRN）5-羟基色胺（5-HT）神经元兴奋性的影响。浴中DA（1-300 microM）的应用在所检查的所有5-HT神经元中产生了浓度依赖性的膜去极化作用。在存在河豚毒素（TTX; 1 microM）和低细胞外钙的情况下，这种作用持续存在。此外，用6,7-二硝基喹喔啉-2,3-二酮（DNQX）和2-氨基-5-膦基戊酸（AP5）阻断离子型谷氨酸受体不能阻止DA引起的去极化，表明它是由直接介导的。 DA对5-HT神经元的影响选择性α1-肾上腺素能受体拮抗剂普拉佐星和WB 4101不能拮抗DA诱导的去极化作用，而非选择性DA受体拮抗剂氟哌啶醇可以拮抗DA引起的去极化作用。另外，选择性的D2样受体激动剂喹吡罗和拮抗剂舒必利分别模拟和阻断了DA诱导的去极化。这些结果表明，DA诱导DRN 5-HT神经元的膜去极化是由D2样DA受体的激活介导的。 DA引起的膜去极化和内向电流与膜电导增加有关。检查DA感应的内向电流的电流-电压（I-V）关系表明，电流幅值随着膜超极化和反极性在-15 mV附近增加。这些数据表明DA诱导DRN 5-HT神经元去极化不是由钾电导的降低介导的，而是最有可能由非选择性阳离子电流的激活介导的。

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