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首页> 外文期刊>The European Journal of Neuroscience >Somal positioning and dendritic growth of horizontal cells are regulated by interactions with homotypic neighbors.
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Somal positioning and dendritic growth of horizontal cells are regulated by interactions with homotypic neighbors.

机译：水平细胞的体位和树突状生长受​​同型邻居相互作用的调节。

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摘要

Retinal neurons extend their dendritic fields to achieve a degree of dendritic overlap with homotypic neighbors that is cell-type specific. How these neurons regulate their dendritic growth is unclear. The dendritic field of a retinal horizontal cell varies inversely with horizontal cell density across different strains of mice, suggesting that proximity to neighboring cells regulates dendritic growth. To test this directly, we have employed the Cre-loxP conditional gene targeting strategy to achieve inactivation of Lim1 function in developing horizontal cells. Through this approach, Lim1 function was prevented within a subset of horizontal cells that in turn fail to migrate to the horizontal cell layer and differentiate normally. For those remaining horizontal cells with Lim1 intact (about half of the normal population in these mice), we show that they spread themselves out tangentially and differentiate a dendritic morphology that is essentially normal but for the fact that it has nearly doubled in area. Such larger horizontal cells, sampling from an area of retina containing twice their normal afferent number, differentiate a dendritic field with nearly double the number of higher order branches and terminal clusters. These results demonstrate directly that positioning and dendritic growth are regulated by interactions with homotypic neighbors, whereas afferents instruct the differentiation of dendritic patterning.
机译：视网膜神经元扩展其树突区域，以达到与特定细胞类型的同型邻居树突重叠的程度。这些神经元如何调节其树突状生长尚不清楚。在不同小鼠品系中，视网膜水平细胞的树突场与水平细胞密度成反比，这表明与邻近细胞的接近调节了树突的生长。为了直接测试这一点，我们采用了Cre-loxP条件基因靶向策略，以在发育水平细胞中实现Lim1功能的失活。通过这种方法，在水平细胞子集中阻止了Lim1功能，而水平细胞又无法迁移到水平细胞层并正常分化。对于那些剩下的具有Lim1完整的水平细胞（大约是这些小鼠正常种群的一半），我们显示出它们切向分布，并区分了本质上是正常的树突形态，但实际上其面积几乎增加了一倍。这种较大的水平细胞从视网膜区域取样，其包含两倍于其正常传入数的视网膜区域，从而使树突状区域分化，其高阶分支和末端簇的数量几乎增加了一倍。这些结果直接表明，定位和树突状生长受​​同型邻居相互作用的调节，而传入基因则指示了树突状图案的分化。

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