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首页> 外文期刊>The European Journal of Neuroscience >Molecular diversity of deep short-axon cells of the rat main olfactory bulb.
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Molecular diversity of deep short-axon cells of the rat main olfactory bulb.

机译：大鼠主要嗅球深部短轴突细胞的分子多样性。

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Local circuit GABAergic interneurons comprise the most diverse cell populations of neuronal networks. Interneurons have been characterized and categorized based on their axo-somato-dendritic morphologies, neurochemical content, intrinsic electrical properties and their firing in relation to in-vivo population activity. Great advances in our understanding of their roles have been facilitated by their selective identification. Recently, we have described three major subtypes of deep short-axon cells (dSACs) of the main olfactory bulb (MOB) based on their axo-dendritic distributions and synaptic connectivity. Here, we investigated whether dSACs also display pronounced molecular diversity and whether distinct dSAC subtypes selectively express certain molecules. Multiple immunofluorescent labeling revealed that the most commonly used molecular markers of dSACs (e.g. vasoactive intestinal polypeptide, calbindin and nitric oxide synthase) label only very small subpopulations (< 7%). In contrast, voltage-gatedpotassium channel subunits Kv2.1, Kv3.1b, Kv4.3 and the GABA(A) receptor alpha1 subunit are present in 70-95% of dSACs without showing any dSAC subtype-selective expression. However, metabotropic glutamate receptor type 1alpha mainly labels dSACs that project to the glomerular layer (GL-dSAC subtype) and comprise approximately 20% of the total dSAC population. Analysing these molecular markers with stereological methods, we estimated the total number of dSACs in the entire MOB to be approximately 13 500, which is around a quarter of the number of mitral cells. Our results demonstrate a large molecular heterogeneity of dSACs and reveal a unique neurochemical marker for one dSAC subtype. Based on our results, dSAC subtype-specific genetic modifications will allow us to decipher the role of GL-dSACs in shaping the dynamic activity of the MOB network.
机译：局部回路的GABA能中神经元组成了神经网络中最多样化的细胞群。中间神经元已经根据它们的轴突-树突树突形态，神经化学含量，内在的电特性以及它们与体内种群活动的关系进行了分类。通过选择性识别，促进了我们对它们角色的理解的巨大进步。最近，我们已经描述了主要的嗅球（MOB）的深短轴突细胞（dSACs）的三种主要亚型，基于它们的轴突-树突分布和突触连通性。在这里，我们调查了dSAC是否还显示出明显的分子多样性，以及不同的dSAC亚型是否选择性表达某些分子。多重免疫荧光标记显示，最常用的dSAC分子标记（例如，血管活性肠多肽，钙结合蛋白和一氧化氮合酶）仅标记非常小的亚群（<7％）。相比之下，电压门控钾通道亚基Kv2.1，Kv3.1b，Kv4.3和GABA（A）受体alpha1亚基存在于70-95％的dSAC中，而未显示任何dSAC亚型选择性表达。但是，代谢型谷氨酸受体1α型主要标记dSAC，这些dSAC投射到肾小球层（GL-dSAC亚型），约占dSAC总数的20％。用立体学方法分析这些分子标记，我们估计整个MOB中dSAC的总数约为13 500，约为二尖瓣细胞数量的四分之一。我们的结果证明了dSAC的大分子异质性，并揭示了一种dSAC亚型的独特神经化学标记。根据我们的结果，dSAC亚型特异性的遗传修饰将使我们能够解读GL-dSAC在塑造MOB网络动态活动中的作用。

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