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首页> 外文期刊>The European Journal of Neuroscience >Cell-type-dependent action potentials and voltage-gated currents in mouse fungiform taste buds
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Cell-type-dependent action potentials and voltage-gated currents in mouse fungiform taste buds

机译：小鼠真菌形味蕾中依赖细胞类型的动作电位和电压门控电流

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摘要

Taste receptor cells fire action potentials in response to taste substances to trigger non-exocytotic neurotransmitter release in type II cells and exocytotic release in type III cells. We investigated possible differences between these action potentials fired by mouse taste receptor cells using in situ whole-cell recordings, and subsequently we identified their cell types immunologically with cell-type markers, an IP3 receptor (IP3R3) for type II cells and a SNARE protein (SNAP-25) for type III cells. Cells not immunoreactive to these antibodies were examined as non-IRCs. Here, we show that type II cells and type III cells fire action potentials using different ionic mechanisms, and that non-IRCs also fire action potentials with either of the ionic mechanisms. The width of action potentials was significantly narrower and their afterhyperpolarization was deeper in type III cells than in type II cells. Na+ current density was similar in type II cells and type III cells, but it was significantly smaller in non-IRCs than in the others. Although outwardly rectifying current density was similar between type II cells and type III cells, tetraethylammonium (TEA) preferentially suppressed the density in type III cells and the majority of non-IRCs. Our mathematical model revealed that the shape of action potentials depended on the ratio of TEA-sensitive current density and TEA-insensitive current one. The action potentials of type II cells and type III cells under physiological conditions are discussed.
机译：味觉受体细胞响应于味觉物质激发动作电位，以触发II型细胞中非胞外神经递质的释放和III型细胞中胞吐的释放。我们使用原位全细胞记录研究了小鼠味觉受体细胞激发的这些动作电位之间的可能差异，随后我们通过细胞类型标记，II型细胞的IP3受体（IP3R3）和SNARE蛋白通过免疫学方法鉴定了它们的细胞类型（SNAP-25）用于III型细胞。对这些抗体无免疫反应性的细胞被检查为非IRC。在这里，我们显示了II型细胞和III型细胞使用不同的离子机制激发动作电位，而非IRCs也通过两种离子机制激发动作电位。与II型细胞相比，III型细胞的动作电位宽度明显更窄，超极化后的深度更深。 Na +电流密度在II型细胞和III型细胞中相似，但在非IRC中显着小于其他类型。尽管II型电池和III型电池的向外整流电流密度相似，但是四乙铵（TEA）优先抑制了III型电池和大多数非IRC电池的密度。我们的数学模型表明，动作电位的形状取决于对TEA敏感的电流密度与对TEA不敏感的电流密度之比。讨论了II型细胞和III型细胞在生理条件下的动作电位。

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