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首页> 外文期刊>The European Journal of Neuroscience >Segregated labeling of olfactory bulb projection neurons based on their birthdates
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Segregated labeling of olfactory bulb projection neurons based on their birthdates

机译：基于嗅球投射神经元的出生日期进行标记

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Mitral and tufted cells are the projection neurons of the olfactory bulb (OB). We previously reported that somata location and innervation patterns were different between early- and late-born mitral cells (Imamura etal., 2011). Here, we introduced a plasmid that drives the expression of a GFP gene into the mouse OB using in utero electroporation, and demonstrated that we can deliver the plasmid vectors into distinct subsets of OB projection neurons by changing the timing of electroporation after fertilisation. The electroporation performed at embryonic day (E)10 preferentially labeled mitral cells in the accessory OB and main OB mitral cells in dorsomedial mitral cell layer (MCL). In contrast, the E12 electroporation introduced the plasmid vectors preferentially into main OB mitral cells in the ventrolateral MCL and tufted cells. Combining these data with BrdU injections, we confirmed that E10 and E12 electroporation preferentially labeled early- and late-born projection neurons, respectively. This work introduces a novel method for segregated labeling of mouse olfactory bulb projection neurons based on their birthdates. With this technique we found that early- and late-born projection neurons extend their secondary dendrites in the deep and superficial external plexiform layer (EPL), respectively. Although a similar segregation has been suggested for mitral vs. tufted cell dendrites, we found mitral cells projecting secondary dendrites into the superficial EPL in E12-electroporated main OB. Our observations indicate that timing of neurogenesis regulates not only somata location and innervation patterns but also the laminar organisation of projection neuron dendrites in the EPL.
机译：二尖瓣和簇状细胞是嗅球（OB）的投射神经元。我们先前曾报道，早产和晚期出生的二尖瓣细胞的躯体位置和神经支配模式不同（Imamura等，2011）。在这里，我们介绍了一种使用子宫内电穿孔法驱动GFP基因表达进入小鼠OB的质粒，并证明了我们可以通过改变受精后的电穿孔时机将质粒载体递送到OB投射神经元的不同子集中。胚胎天（E）10进行的电穿孔优先标记了辅助OB中的二尖瓣细胞和背囊二尖瓣细胞层（MCL）中的主要OB二尖瓣细胞。相反，E12电穿孔将质粒载体优先引入腹侧MCL和簇状细胞的主要OB二尖瓣细胞中。将这些数据与BrdU注射液结合使用，我们确认E10和E12电穿孔分别优先标记了早产和晚期出生的投射神经元。这项工作介绍了一种新的方法，用于基于小鼠嗅球投射神经元的出生日期进行标记。通过这项技术，我们发现早产和晚产的投射神经元分别在深层和浅层外部丛状层（EPL）中扩展其次级树突。尽管有人建议对二尖瓣和簇状细胞树突进行类似的分离，但我们发现二尖瓣细胞在E12电穿孔的主OB中将次生树突投射到浅表EPL中。我们的观察表明，神经发生的时间不仅调节躯体的位置和神经支配方式，而且还调节EPL中投射神经元树突的层状组织。

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